Effects of sleep fragmentation on sleep and markers of inflammation in mice.
Effects of sleep fragmentation on sleep and markers of inflammation in mice.
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DOI：
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发表时间：
2014-02
影响因子：
0.8
通讯作者：
R. Trammell;S. Verhulst;L. Toth
中科院分区：
文献类型：
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作者：
R. Trammell;S. Verhulst;L. Toth
在我们的社会中，由于工作责任、照顾或生活方式的选择，许多人都经历过睡眠减少和中断。描述急性和慢性睡眠中断对健康的影响对于提高对这些普遍问题的认识和制定干预措施至关重要。为了提供一个研究与睡眠不足相关的健康影响和潜在病理生理机制的平台，我们开发并描述了一种在实验室小鼠中制造慢性睡眠中断的方法。我们用这种方法评估了3个睡眠片段（SF）持续时间对雄性C57BL/6J小鼠睡眠恢复和血液和肺分析物浓度的影响。在连续4天的光照（嗜睡）阶段，将小鼠置于环境控制的房间中，每天分别暴露于6、12、24小时或12小时的自动SF。在每3次SF持续时间后允许不间断睡眠时，睡眠时间、慢波振幅或回合长度明显更高。然而，在随后的12到24小时内，小鼠并没有完全恢复失去的慢波睡眠，而是保持了净睡眠损失。光期SF与血清和肺部某些炎症物质水平的显著变化相关，但这些变化不一致或持续。这些数据表明，急性光期SF可导致小鼠持续的睡眠债务，并可能破坏血清和肺部的炎症稳态。
Many people in our society experience curtailment and disruption of sleep due to work responsibilities, care-giving, or life style choice. Delineating the health effect of acute and chronic disruptions in sleep is essential to raising awareness of and creating interventions to manage these prevalent concerns. To provide a platform for studying the health impact and underlying pathophysiologic mechanisms associated with inadequate sleep, we developed and characterized an approach to creating chronic disruption of sleep in laboratory mice. We used this method to evaluate how 3 durations of sleep fragmentation (SF) affect sleep recuperation and blood and lung analyte concentrations in male C57BL/6J mice. Mice housed in environmentally controlled chambers were exposed to automated SF for periods of 6, 12, or 24 h or for 12 h daily during the light (somnolent) phase for 4 sequential days. Sleep time, slow-wave amplitude, or bout lengths were significantly higher when uninterrupted sleep was permitted after each of the 3 SF durations. However, mice did not recover all of the lost slow-wave sleep during the subsequent 12- to 24-h period and maintained a net loss of sleep. Light-phase SF was associated with significant changes in serum and lung levels of some inflammatory substances, but these changes were not consistent or sustained. The data indicate that acute light-phase SF can result in a sustained sleep debt in mice and may disrupt the inflammatory steady-state in serum and lung.