Ranking skin‐sensitizing hair dye molecules according to their potency by the use of human cells
Ranking skin‐sensitizing hair dye molecules according to their potency by the use of human cells
复制标题
使用人体细胞根据其功效对皮肤敏感染发剂分子进行排名
作者：
J. Hennen;B. Blömeke
在没有动物试验的情况下确定染发剂成分的皮肤致敏潜力（效力）对于定量风险评估至关重要。除了对苯二胺（PPD）和甲苯-2，5-二胺（对甲苯二胺[PTD]）这两种常用的前体和氧化性染发剂相关接触性皮炎中的主要过敏原之外，目前使用的成分超过100种，包括前体对氨基苯酚。对氨基苯酚与1-萘酚、间氨基苯酚或间苯二酚等成色剂一起使用，以产生不同的色调。动物试验表明，间氨基苯酚、对氨基苯酚、1-萘酚和间苯二酚是接触致敏剂，被消费者安全科学委员会归类为强接触致敏剂。然而，尽管前体对氨基苯酚和偶合剂-氨基苯酚经常在斑贴测试患者中引起斑贴测试反应，患病率在1.4%至11%之间变化，但对1-萘酚或间苯二酚的斑贴测试反应很少见，尽管这些偶合剂也经常使用。除了暴露水平和潜在交叉反应模式的差异外，人体致敏效力的差异可能是患者反应差异的原因。我们以前显示了我们的共培养模型（COCAT）的能力，包括人类细胞系，即HaCaT角质形成细胞和THP-1细胞作为替代树突状细胞，根据其效力的基础上评估的浓度诱导共刺激分子CD 86和/或adhesionmolecule CD 54共培养的THP-1细胞的显着上调的PPD，PTD，和2-甲氧基甲基-对苯二胺排名。本文研究了对氨基苯酚、间氨基苯酚、1-萘酚和间苯二酚在该模型中的致敏效能。方法
The determination of the skin sensitization potential (potency) of hair dye ingredients without animal testing is crucial for their quantitative risk assessment. Besides p-phenylenediamine (PPD) and toluene-2, 5-diamine (p-toluylenediamine [PTD]), which are 2 commonly used precursors and major allergens in oxidative hair dye-associated contact dermatitis, >100 ingredients are currently used, including the precursor p-aminophenol. p-Aminophenol is used together with couplers such as 1-naphthol, m-aminophenol or resorcinol to create different shades. Animal tests have indicated that m-aminophenol, p-aminophenol, 1-naphthol and resorcinol are contact sensitizers, being classified as strong contact sensitizers by the Scientific Committee on Consumer Safety. However, whereas the precursor p-aminophenol and the couplerm-aminophenol frequently elicit patch test reactions in patch tested patients, with prevalences varying between 1.4% and 11%, patch test reactions to 1-naphthol or resorcinol are rare, although these couplers are also frequently used. Besides differences in exposure level and potential cross-reactivity patterns, differences in sensitization potency in humansmay account for such different responses in patients. We previously showed the capacity of our coculture model (COCAT) consisting of human cell lines, that is, HaCaT keratinocytes and THP-1 cells as surrogate dendritic cells, to rank PPD, PTD, and 2-methoxymethyl-p-phenylenediamine according to their potency based on the evaluation of the concentration inducing significant upregulation of the costimulatory molecule CD86 and/or adhesionmolecule CD54 on cocultured THP-1 cells. Here, we studied the sensitization potency of p-aminophenol,m-aminophenol, 1-naphthol and resorcinol in thismodel. METHODS