False-positive rate determination of protein target discovery using a covalent modification- and mass spectrometry-based proteomics platform.
False-positive rate determination of protein target discovery using a covalent modification- and mass spectrometry-based proteomics platform.
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DOI：
10.1007/s13361-013-0754-2
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发表时间：
2014-01
影响因子：
3.2
通讯作者：
Fitzgerald, Michael C.
中科院分区：
文献类型：
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作者：
Strickland, Erin C.;Geer, M. Ariel;Hong, Jiyong;Fitzgerald, Michael C.
Detection and quantitation of protein-ligand binding interactions is important in many areas of biological research. The Stability of Proteins from Rates of Oxidation (SPROX) technique is an energetics-based technique for identifying the proteins targets of ligands in complex biological mixtures. Knowing the false positive rate of protein target discovery in proteome-wide SPROX experiments is important for the correct interpretation of results. Reported here are the results of a control SPROX experiment in which chemical denaturation data is obtained on the proteins in two samples that originated from the same yeast lysate, as would be done in a typical SPROX experiment except that one sample would be spiked with the test ligand. False positive rates of 1.2–2.2% and <0.8% are calculated for SPROX experiments using Q-TOF and orbitrap mass spectrometer systems, respectively. Our results indicate that the false positive rate is largely determined by random errors associated with the mass spectral analysis of the isobaric mass tag (e.g., iTRAQ®) reporter ions used for peptide quantitation. Our results also suggest that technical replicates can be used to effectively eliminate such false positives that result from this random error, as is demonstrated in a SPROX experiment to identify yeast protein targets of the drug, manassantin A. The impact of ion purity in the tandem mass spectral analyses and of background oxidation on the false positive rate of protein target discovery using SPROX is also discussed.
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影响因子：
4.4
作者：
DeArmond, Patrick D.;Xu, Ying;Strickland, Erin C.;Daniels, Kyle G.;Fitzgerald, Michael C.
通讯作者：
Fitzgerald, Michael C.
影响因子：
48
作者：
Park, CW;Marqusee, S
通讯作者：
Marqusee, S
影响因子：
8
作者：
Chang, Youngil;Schlebach, Jonathan P.;Park, Chiwook
通讯作者：
Park, Chiwook
DOI：
10.1038/nsb1001-879
发表时间：
2001-10-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Ghaemmaghami, S;Oas, TG
通讯作者：
Oas, TG
影响因子：
14.8
作者：
通讯作者：
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