Assembly of single bacteriorhodopsin trimers in bilayer nanodiscs
Assembly of single bacteriorhodopsin trimers in bilayer nanodiscs
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DOI：
10.1016/j.abb.2006.03.013
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发表时间：
2006-06-15
影响因子：
3.9
通讯作者：
Sligar, Stephen G.
中科院分区：
文献类型：
--
作者：
Bayburt, Timothy H.;Grinkova, Yelena V.;Sligar, Stephen G.
纳米盘是一种控制大小的磷脂双层组装体，用于将细菌视紫红质（bR）自组装成单个三聚体。以最佳bR自组装到纳米盘和磷脂化学计量产生含有三个bR分子的颗粒。溶液小角x射线散射分析表明，细菌视紫红质包裹在盘状磷脂双层结构中。三聚体的形成，正如视网膜吸收带可见的圆二色性所证明的那样，在纳米圆盘中，在特定的尺寸阈值下，有利于三聚体的形成，这表明一个关键的双层面积或脂质量是维持天然低聚物状态所必需的。在组装过程中，脂质与溴的比例也是决定寡聚化状态的重要因素。这些纳米级双分子层为理解和控制寡聚整体膜蛋白的组装提供了机会，寡聚整体膜蛋白对大分子识别和细胞信号传导至关重要。(c) 2006爱思唯尔公司版权所有。
Nanodiscs, phospholipid bilayer assemblies of controlled size, were used to self-assemble bacteriorhodopsin (bR) into single trimers. Self-assembly at optimal bR to Nanodisc and phospholipid stoichiometry yielded particles containing three bR molecules. Analysis of solution small angle X-ray scattering indicated that bacteriorhodopsin is embedded in a discoidal phospholipid bilayer structure. Formation of trimers, as evidenced by visible circular dichroism of the retinal absorbance bands, is facilitated in Nanodiscs at a specific size threshold, suggesting that a critical bilayer area or amount of lipid is necessary to maintain a native oligomeric state. The lipid to bR ratio in the assembly process was also found to be an important factor in determining oligomerization state. These nanoscale bilayers offer the opportunity to understand and control the assembly of oligomeric integral membrane proteins critical to macromolecular recognition and cellular signaling. (c) 2006 Elsevier Inc. All rights reserved.