Effects of titanium surface anodization with CaP incorporation on human osteoblastic response.
Effects of titanium surface anodization with CaP incorporation on human osteoblastic response.
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DOI：
10.1016/j.msec.2013.01.002
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发表时间：
2013-05-01
期刊：
影响因子：
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通讯作者：
Dechichi P
中科院分区：
文献类型：
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作者：
Oliveira NC;Moura CC;Zanetta-Barbosa D;Mendonça DB;Cooper L;Mendonça G;Dechichi P
在这项研究中，我们研究了与酸蚀刻表面（Porous®）和机械加工表面（Screw®）相比，磷酸钙（CaP）掺入阳极氧化（Vulcano®）是否在3天和7天后增强生长因子分泌、成骨细胞特异性基因表达和细胞活力。结果显示，与螺钉相比，多孔和Vulcano在第7天的细胞活力显著（p=0.005）。在同一时间点，发现所有表面的runt相关转录因子2（Runx 2）、碱性磷酸酶（ALP）和骨唾液蛋白（BSP）表达存在显著差异（p<0.05），但Porous和Vulcano的诱导倍数更大。转化生长因子β1（TGF-β1）和骨形态发生蛋白2（BMP-2）的分泌在任何实验时间均不受表面处理的显著影响（p>0.05）。虽然没有发现生长因子分泌和Runx 2表达的显着相关性，该基因和ALP/BSP表达之间的显着正相关性表明，它们的强关联是独立的表面类型。CaP的掺入影响的生物学参数评价类似于表面只是酸蚀刻。这里提出的结果支持的意见，粗糙度也可能发挥重要作用，在确定细胞的反应。
In this study we investigated whether anodization with calcium phosphate (CaP) incorporation (Vulcano®) enhances growth factors secretion, osteoblast-specific gene expression, and cell viability, when compared to acid etched surfaces (Porous®) and machined surfaces (Screw®) after 3 and 7 days. Results showed significant cell viability for Porous and Vulcano at day 7, when compared with Screw (p=0.005). At the same time point, significant differences regarding runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP) and bone sialoprotein (BSP) expression were found for all surfaces (p<0.05), but with greater fold induction for Porous and Vulcano. The secretion of transforming growth factor β1 (TGF-β1) and bone morphogenetic protein 2 (BMP-2) was not significantly affected by surface treatment in any experimental time (p>0.05). Although no significant correlation was found for growth factors secretion and Runx2 expression, a significant positive correlation between this gene and ALP/BSP expression showed that their strong association is independent on the type of surface. The incorporation of CaP affected the biological parameters evaluated similar to surfaces just acid etched. The results presented here support the observations that roughness also may play an important role in determining cell response.