Nucleotide sequence of the human c‐myc locus: provocative open reading frame within the first exon.
Nucleotide sequence of the human c‐myc locus: provocative open reading frame within the first exon.
复制标题
人类 c-myc 位点的核苷酸序列：第一个外显子内的激发性开放阅读框。
作者：
C. Gazin;S. Dupont de Dinechin;A. Hampe;J. Masson;P. Martin;D. Stéhelin;F. Galibert
通过Maxam和吉尔伯特的方法推导出了含有完整人类c-myc基因的λ重组体的HindIII-EcoRI DNA片段（8 kbp长）的核苷酸序列。该片段编码复杂的c-myc基因座，该序列提供了与2.7 kb长的c-myc转录物相关的信息。虽然外显子2和3编码的48-K蛋白与病毒p110 gag-myc蛋白的myc结构域同源，但第一个外显子（其具有一个大的开放阅读框，以供体剪接位点上游的终止密码子结尾）可以自己编码20-K蛋白。推测该推定蛋白质对外显子2和3的表达的调节的作用。
The nucleotide sequence of a HindIII‐EcoRI DNA fragment, 8 kbp long, of a lambda recombinant containing the whole human c‐myc gene has been deduced by the method of Maxam and Gilbert. This fragment encodes the complex c‐myc locus and the sequence provides information relative to the 2.7 kb long c‐myc transcript. It appears that although exons 2 and 3 would code for a 48‐K protein homologous to the myc domain of the viral p110 gag‐myc protein, the first exon, which has a large open reading frame ending with a stop codon just upstream from the donor splice site, could code on its own for a 20‐K protein. Speculations about the role of that putative protein on the regulation of the expression of exons 2 and 3 are made.