Biochemical requirements for PCBCK1 kinase activity, the Pneumocystis carinii MEKK involved in cell wall integrity.
Biochemical requirements for PCBCK1 kinase activity, the Pneumocystis carinii MEKK involved in cell wall integrity.
复制标题
生化要求PCBCK1激酶活性，卡氏肺囊虫MEKK参与细胞壁完整性。
DOI：
10.1016/j.femsle.2004.04.021
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
ThomasJr,CharlesF
中科院分区：
文献类型：
--
作者：
Vohra,PawanK;Sanyal,Bharati;ThomasJr,CharlesF
真菌细胞壁组装是一个复杂的过程，涉及多个酶和协调的信号通路。细胞壁完整性MAPK途径通过调节葡聚糖合成在高温条件下稳定真菌细胞壁。上游激酶BCK 1是该途径的关键组分。肺炎是一个重要的原因死亡的真菌机会性病原体肺孢子虫在免疫功能低下的国家，特别是与艾滋病毒感染。我们以前已经表明，PCBCK 1功能在酵母细胞壁完整性途径作为一种功能性蛋白激酶。激酶对酶的功能有特殊的要求，这在真菌中还没有研究过。在这里，我们研究了PCBCK 1激酶活性的生化要求表达在酿酒酵母bck 1 Δ酵母。PCBCK1需要10 mM MgCl2、pH 6、温度30 °C和10 μM ATP才能获得激酶活性。干扰肺孢子虫细胞壁完整性途径是药物开发的一个有吸引力的目标，因为葡聚糖合成机制在人类中不存在。
Fungal cell wall assembly is a complicated process involving multiple enzymes and coordinated signaling pathways. The cell wall integrity MAPK pathway acts to stabilize the fungal cell wall during conditions of elevated temperature by regulation of glucan synthesis. The upstream kinase, BCK1, is a critical component of this pathway. Pneumonia is a significant cause of death from the fungal opportunistic pathogenPneumocystisin immunocompromised states, especially with HIV infection. We have previously shown that PCBCK1 functions in the cell wall integrity pathway in yeast as a functional protein kinase. Kinases have specific requirements for enzymatic function which have not been investigated in fungi. Here we examine the biochemical requirements for PCBCK1 kinase activity expressed inSaccharomyces cerevisiae bck1Δ yeast. PCBCK1 requires 10 mM MgCl2, pH 6, temperature 30 °C, and 10 μM ATP for kinase activity. Interference of thePneumocystiscell wall integrity pathway is an attractive target for drug development since glucan synthesis machinery is not present in humans.