Immobilized MutS-Mediated Error Removal of Microchip-Synthesized DNA.
Immobilized MutS-Mediated Error Removal of Microchip-Synthesized DNA.
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DOI：
10.1007/978-1-4939-6343-0_17
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发表时间：
2017
影响因子：
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通讯作者：
Wen Wan;Dongmei Wang;Xiaolian Gao;Jiong Hong
中科院分区：
文献类型：
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作者：
Wen Wan;Dongmei Wang;Xiaolian Gao;Jiong Hong
Applications of microchip-synthesized oligonucleotides for de novo gene synthesis are limited primarily by their high error rates. The mismatch binding protein MutS, which can specifically recognize and bind to mismatches, is one of the cheapest tools for error correction of synthetic DNA. Here, we describe a protocol for removing errors in microchip-synthesized oligonucleotides and for the assembly of DNA segments using these oligonucleotides. This protocol can also be used in traditional de novo gene DNA synthesis.
