Transmissibility of Cucumber mosaic virus by Aphis gossypii correlates with viral accumulation and is affected by the presence of its satellite RNA
Transmissibility of Cucumber mosaic virus by Aphis gossypii correlates with viral accumulation and is affected by the presence of its satellite RNA
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DOI：
10.1094/phyto.2000.90.10.1068
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发表时间：
2000-10-01
期刊：
影响因子：
3.2
通讯作者：
García-Arenal, F
中科院分区：
文献类型：
--
作者：
Escriu, F;Perry, KL;García-Arenal, F
卫星RNA（satRNA）与番茄中的黄瓜花叶病毒（CMV）相关，最常引起坏死植物的严重流行，并且与特定的宿主症状无关。为了更好地了解CMV田间种群动态，进行了棉蚜传毒的实验室研究。卫星RNA的存在与感染植物中较低的病毒浓度和卫星RNA感染植物的传播效率降低相关。在CMV感染的番茄中，病毒浓度和传播效率与非坏死性卫星RNA的差异比坏死性卫星RNA更大。卫星RNA对病毒积累的负面影响可以部分地解释CMV +卫星RNA的田间传播和恢复的减少。蚜虫的行为不同，探测不容易与CMV +坏死satRNA感染的植物相比，含有nonnecrogenic satRNA的植物。蚜虫介导的satRNA无CMV感染时，观察到在测试植物蚜虫喂源植物含有CMV + nonnecrogenic satRNA;没有可比的satRNA无测试植物发生时，蚜虫喂源植物含有necrogenic satRNA。这些结果表明，与传播相关的因素可能是CMV及其卫星RNA自然种群进化的决定因素。
Satellite RNAs (satRNAs) are associated with Cucumber mosaic virus (CMV) in tomato, most often causing severe epidemics of necrotic plants, and not associated with specific host symptoms. Laboratory studies on virus transmission by the aphid vector Aphis gossypii were performed to better understand the dynamics of field populations of CMV. The presence of satRNAs correlated with lower concentrations of virus in infected plants and with a decrease in the efficiency of transmission from satRNA-infected plants. Both the concentration of virus in CMV-infected tomato and the efficiency of transmission varied more extensively with nonnecrogenic satRNAs than with necrogenic satRNAs. A negative effect of satRNAs on virus accumulation can account, in part, for a decrease in the field transmission and recovery of CMV + satRNAs. Aphids behaved differently and probed less readily on plants infected with CMV + necrogenic satRNAs compared with plants containing nonnecrogenic satRNAs. Aphid-mediated satRNA-free CMV infections were observed in test plants when aphids were fed on source plants containing CMV + nonnecrogenic satRNA; no comparable satRNA-free test plants occurred when aphids were fed on source plants containing necrogenic satRNAs. These results indicate that factors associated with transmission can be a determinant in the evolution of natural populations of CMV and its satRNA.