Metabolic engineering of corynebacterium glutamicum for fuel ethanol production under oxygen-deprivation conditions
Metabolic engineering of corynebacterium glutamicum for fuel ethanol production under oxygen-deprivation conditions
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DOI：
10.1159/000086705
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发表时间：
2004-01-01
影响因子：
1.2
通讯作者：
Yukawa, H
中科院分区：
文献类型：
--
作者：
Inui, M;Kawaguchi, H;Yukawa, H
谷氨酸棒杆菌的中心代谢途径被工程化以产生乙醇。构建了表达丙酮酸脱羧酶（pdc）和乙醇脱氢酶（adhB）基因的运动发酵单胞菌重组菌株。这两个基因都在C. glutamicum ldhA启动子在C.谷氨酸，导致在缺氧条件下，在以不存在细胞生长为特征的过程中从葡萄糖显著产率乙醇。向反应混合物中添加痕量丙酮酸盐诱导乙醇生产速率增加2倍。当加入乙醛时观察到类似的效果。乳酸脱氢酶（ldhA）基因的破坏导致比野生型高3倍的乙醇产率，而没有乳酸产生。此外，磷酸烯醇式丙酮酸羧化酶（ppc）和ldhA基因的失活揭示了显着量的乙醇生产和琥珀酸的显着减少，而没有任何乳酸的生产，当丙酮酸添加。由于该反应在没有细胞生长的情况下发生，因此乙醇体积生产率与产乙醇C.谷氨酸在缺氧条件下的过程。这些观察结果证实了C.谷氨酸与缺氧代谢流相关。版权所有（C）2004 S. Karger AG，巴塞尔。
The central metabolic pathway of Corynebacterium glutamicum was engineered to produce ethanol. A recombinant strain which expressed the Zymomonas mobilis genes coding for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) was constructed. Both genes placed under the control of the C. glutamicum ldhA promoter were expressed at high levels in C. glutamicum, resulting, under oxygen-deprivation conditions, in a significant yield of ethanol from glucose in a process characterized by the absence of cellular growth. Addition of pyruvate in trace amounts to the reaction mixture induced a 2-fold increase in the ethanol production rate. A similar effect was observed when acetaldehyde was added. Disruption of the lactate dehydrogenase ( ldhA) gene led to a 3-fold higher ethanol yield than wild type, with no lactate production. Moreover, inactivation of the phosphoenolpyruvate carboxylase (ppc) and ldhA genes revealed a significant amount of ethanol production and a dramatic decrease in succinate without any lactate production, when pyruvate was added. Since the reaction occurred in the absence of cell growth, the ethanol volumetric productivity increased in proportion to cell density of ethanologenic C. glutamicum in a process under oxygen-deprivation conditions. These observations corroborate the view that intracellular NADH concentrations in C. glutamicum are correlated to oxygen-deprived metabolic flows. Copyright (C) 2004 S. Karger AG, Basel.