Sister chromatid junctions in the hyperthermophilic archaeon Sulfolobus solfataricus
Sister chromatid junctions in the hyperthermophilic archaeon Sulfolobus solfataricus
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DOI：
10.1038/sj.emboj.7601529
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发表时间：
2007-02-07
期刊：
影响因子：
11.4
通讯作者：
Bell, Stephen D.
中科院分区：
文献类型：
--
作者：
Robinson, Nicholas P.;Blood, Katherine A.;Bell, Stephen D.
尽管原核生物表现出细菌和真核生物相似特征的有趣组合，但尚不清楚这些原核细胞在细胞分裂过程之前如何分离其染色体。在我们的第三个复制起点在古菌硫磺硫化叶菌的分析过程中，我们确定和crossing姐妹染色单体连接在这个原核生物。这种配对似乎是介导的hemicatenane样结构，我们提供的证据表明，这些路口坚持在复制和postreplicative细胞。这些数据，结合荧光原位杂交分析，表明硫化叶菌染色体有一个显着的postreplicative姐妹染色单体突触，比细菌染色体分离机制的情况下，更让人想起真核生物。
Although the Archaea exhibit an intriguing combination of bacterial- and eukaryotic-like features, it is not known how these prokaryotic cells segregate their chromosomes before the process of cell division. In the course of our analysis of the third replication origin in the archaeon Sulfolobus solfataricus, we identify and characterise sister chromatid junctions in this prokaryote. This pairing appears to be mediated by hemicatenane-like structures, and we provide evidence that these junctions persist in both replicating and postreplicative cells. These data, in conjunction with fluorescent in situ hybridisation analyses, suggest that Sulfolobus chromosomes have a significant period of postreplicative sister chromatid synapsis, a situation that is more reminiscent of eukaryotic than bacterial chromosome segregation mechanisms.