cDNA cloning of rat proteasome subunit RC7‐I, a homologue of yeast PRE1 essential for chymotrypsin‐like activity
cDNA cloning of rat proteasome subunit RC7‐I, a homologue of yeast PRE1 essential for chymotrypsin‐like activity
复制标题
大鼠蛋白酶体亚基 RC7-I 的 cDNA 克隆，它是胰凝乳蛋白酶样活性所必需的酵母 PRE1 的同源物
DOI：
10.1016/0014-5793(93)80482-a
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发表时间：
1993
期刊：
影响因子：
3.5
通讯作者：
A. Ichihara
中科院分区：
文献类型：
--
作者：
C. Nishimura;T. Tamura;F. Tokunaga;Keiji Tanaka;A. Ichihara
编码大鼠肝癌细胞20s蛋白酶体新亚基RC7-I的cDNA的核苷酸序列已被确定。该多肽由201个氨基酸残基组成，计算分子量为22912，等电点为7.25。通过蛋白质化学分析，发现rc7 - 1的部分氨基酸序列约有80%与cDNA序列的推断非常一致。计算机分析表明，rc7 - 1属于蛋白酶体的β型亚群，与古细菌蛋白酶体的β-亚基相似，与蛋白酶体基因家族的α型亚基明显不同。研究发现，RC7-I的整体结构与酵母PRE1的结构相似，而PRE1是酵母促凝胰蛋白酶活性所必需的。
The nucleotide sequence of a cDNA that encodes a new subunit, named RC7-I, of the 20 S proteasome of rat hepatoma cells has been determined. The polypeptide predicted from the open reading frame consists of 201 amino acid residues with a calculated molecular weight of 22,912 and isoelectric point of 7.25. Approximately 80% of the partial amino acid sequences of several fragments of RC7-I, determined by protein chemical analyses, were found to be in excellent accordance with those deduced from the cDNA sequence. Computer analysis showed that RC7-I belongs to the β-type subgroup of proteasomes with similarity to the β-subunit of the archaebacterial proteasome, differing clearly from α-type subunits of the proteasome gene family. The overall structure of RC7-I was found to be homologous to that of yeast PRE1, which is necessary for chymotryptic activity.