Antigenic and genetic analysis of H3N8 influenza viruses isolated from horses in Japan and Mongolia, and imported from Canada and Belgium during 2007-2010
Antigenic and genetic analysis of H3N8 influenza viruses isolated from horses in Japan and Mongolia, and imported from Canada and Belgium during 2007-2010
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2007-2010年从日本和蒙古马匹分离以及从加拿大和比利时进口的H3N8流感病毒的抗原和基因分析
DOI：
10.1007/s00705-011-1000-5
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发表时间：
2011
影响因子：
2.7
通讯作者：
H. Kida
中科院分区：
文献类型：
--
作者：
Masayuki Motoshima;M. Okamatsu;S. Asakura;Saya Kuribayashi;S. Sengee;Damdinjav Batchuluun;Mika Ito;Y. Maeda;M. Eto;Y. Sakoda;R. Sodnomdarjaa;H. Kida
从感染的马中分离到A/equine/金泽/1/2007（H3 N8）、A/equine/北海道/I828/2008（H3 N8）和A/equine/蒙古/1/2008（H3 N8）。A/equine/Yokohama/aq 19/2009（H3 N8）和A/equine/Yokohama/aq 13/2010（H3 N8）是从在日本横滨动物检疫局检查的从加拿大和比利时进口的马中分离的。在本研究中，这五个分离株进行了遗传和抗原性分析。血凝素（HA）和神经氨酸酶（NA）基因的系统发育分析表明，从日本和加拿大进口的3个分离株属于佛罗里达亚系的同一个分支，分支1;从蒙古和比利时进口的分离株属于佛罗里达亚系的另一个分支，分支2。A/马/金泽/1/2007（H3 N8）HA的一组单克隆抗体与5个分离株的反应模式表明，这些病毒的HA在抗原性上彼此相似，并与参考毒株A/马/拉普拉塔/1/1993（H3 N8）和A/马/Avesta/1/1993（H3 N8）相似。目前的研究结果表明，在马中H3 N8流感病毒之间没有积累广泛的抗原变异。
A/equine/Kanazawa/1/2007 (H3N8), A/equine/Hokkaido/I828/2008 (H3N8) and A/equine/Mongolia/1/2008 (H3N8) were isolated from infected horses. A/equine/Yokohama/aq19/2009 (H3N8) and A/equine/Yokohama/aq13/2010 (H3N8) were isolated from horses imported from Canada and Belgium examined at the Animal Quarantine Service in Yokohama, Japan. In the present study, these five isolates were genetically and antigenically analyzed. Phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed that three isolates from horses in Japan and imported from Canada belonged to the same branch, clade 1 of the Florida sublineage, while the isolates from horses in Mongolia and imported from Belgium belonged to another branch, clade 2 of the Florida sublineage. Reactivity patterns of a panel of monoclonal antibodies to the HA of A/equine/Kanazawa/1/2007 (H3N8) with the five isolates indicate that the HAs of these viruses were antigenically similar to each other and to the reference strains A/equine/La Plata/1/1993 (H3N8) and A/equine/Avesta/1/1993 (H3N8). The present findings indicate that extensive antigenic variation has not accumulated among H3N8 influenza viruses in horses.
影响因子：
3.7
作者：
Ha, Y;Stevens, DJ;Wiley, DC
通讯作者：
Wiley, DC
影响因子：
3.7
作者：
KIDA, H;BROWN, LE;WEBSTER, RG
通讯作者：
WEBSTER, RG