Localization of Tektin 1 at Both Acrosome and Flagella of Mouse and Bull Spermatozoa
Localization of Tektin 1 at Both Acrosome and Flagella of Mouse and Bull Spermatozoa
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DOI：
10.2108/zsj.31.101
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发表时间：
2014-02
期刊：
影响因子：
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通讯作者：
S. Oiki;Erina Hiyama;T. Gotoh;H. Iida
中科院分区：
文献类型：
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作者：
S. Oiki;Erina Hiyama;T. Gotoh;H. Iida
Tektins（TEKT）是纤毛、鞭毛、基体和中心粒中微管的组成性丝状蛋白。在哺乳动物中，已经在睾丸和精子中鉴定出五种TEKT（TEKT 1、2、3、4和5）。除TEKT 1外，已报道这些TEKT存在于精子中，主要定位于鞭毛的轴丝周围结构，即，线粒体和外部致密纤维。在本研究中，我们生产了针对TEKT 1的抗体，以检查TEKT 1在小鼠、公牛和大鼠精子中的定位。通过免疫印迹分析和免疫荧光显微镜，我们发现TEKT 1存在于精子鞭毛和顶体帽的顶端区域在所有这些物种的精子。小鼠精子体外顶体反应后，顶体相关TEKT 1消失。这些观察结果表明TEKT 1作为精子头部的细胞骨架元件或作为参与顶体相关现象（如顶体反应）的分子的另一个潜在作用。
Tektins (TEKTs) are constitutive filamentous proteins of microtubules in cilia, flagella, basal bodies, and centrioles. In mammals, five TEKTs (TEKT1, 2, 3, 4, and 5) have been identified in testis and spermatozoa. With the exception of TEKT1, these TEKTs have been reported to be present in spermatozoa with predominant localization at the peri-axoneme structures of flagella, i.e., mitochondria and outer dense fibers. In the present study, we produced an antibody against TEKT1 to examine the localization of TEKT1 in mouse, bull, and rat spermatozoa. By immunoblot analyses and immunofluorescence microscopy, we found TEKT1 to be present in sperm flagella and at the apical region of acrosome cap in spermatozoa of all these species. Acrosome-associated TEKT1 disappeared after in vitro acrosome reaction in mouse spermatozoa. These observations suggest another potential role for TEKT1 as a cytoskeletal element in the sperm head, or as a molecule involved in acrosome-related phenomena, such as acrosome reaction.