An efficient in vitro plantlet regeneration from shoot tip cultures of Curculigo latifolia, a medicinal plant.
An efficient in vitro plantlet regeneration from shoot tip cultures of Curculigo latifolia, a medicinal plant.
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DOI：
10.1155/2014/275028
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发表时间：
2014
影响因子：
--
通讯作者：
Abdullah TL
中科院分区：
文献类型：
--
作者：
Babaei N;Abdullah NA;Saleh G;Abdullah TL
采用茎尖培养的方法，建立了宽叶石蒜的离体快繁体系。在附加不同浓度和组合的噻苯隆和吲哚-3-丁酸的MS培养基上，获得了宽叶石蒜茎尖的直接再生和间接头皮诱导。培养10周和14周后，在添加噻苯隆（0.5 mg L-1）和吲哚-3-丁酸（0.25 mg L-1）的MS培养基上，获得了直接再生的最大反应，即外植体产生芽的百分比、芽数和芽长度。吲哚-3-丁酸与噻苯隆组合对芽再生表现出协同效应。茎尖能够在含有2 mg L−1噻苯隆的培养基上从外植体的基部末端诱导最大量的头皮。培养物显示，芽数，芽长，头皮大小显着增加后，14周的文化。将芽转移到不含生长调节剂的MS培养基上导致最高的根诱导率和更长的根，而添加0.25 mg L−1 IBA的培养基产生更多的根。
A procedure was developed for in vitro propagation of Curculigo latifolia through shoot tip culture. Direct regeneration and indirect scalp induction of Curculigo latifolia were obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explants producing shoot, shoot number, and shoot length was obtained on MS medium supplemented with combination of thidiazuron (0.5 mg L−1) and indole-3-butyric acid (0.25 mg L−1) after both 10 and 14 weeks of cultures. Indole-3-butyric acid in combination with thidiazuron exhibited a synergistic effect on shoot regeneration. The shoot tips were able to induce maximum scalp from basal end of explants on the medium with 2 mg L−1 thidiazuron. Cultures showed that shoot number, shoot length, and scalp size increased significantly after 14 weeks of culture. Transferring of the shoots onto the MS medium devoid of growth regulators resulted in the highest percentage of root induction and longer roots, while medium supplemented with 0.25 mg L−1 IBA produced more numbers of roots.