Unveiling Cell Surface and Type IV Secretion Proteins Responsible for Archaeal Rudivirus Entry
Unveiling Cell Surface and Type IV Secretion Proteins Responsible for Archaeal Rudivirus Entry
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DOI：
10.1128/jvi.01495-14
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发表时间：
2014-09-01
影响因子：
5.4
通讯作者：
Peng, Xu
中科院分区：
文献类型：
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作者：
Deng, Ling;He, Fei;Peng, Xu
硫化叶菌突变体抗古细菌裂解病毒硫化叶菌杆状病毒2（SIRV2）的分离，并确定在两个基因簇，簇sso3138到sso3141和簇sso2386和sso2387，分别编码细胞表面和IV型分泌蛋白的突变。通过遗传互补证实了突变参与抗性。病毒进入突变体的阻断通过缺乏早期基因转录来证明，这强烈支持蛋白质在SIRV2进入中的作用的想法。
Sulfolobus mutants resistant to archaeal lytic virus Sulfolobus islandicus rod-shaped virus 2 (SIRV2) were isolated, and mutations were identified in two gene clusters, cluster sso3138 to sso3141 and cluster sso2386 and sso2387, encoding cell surface and type IV secretion proteins, respectively. The involvement of the mutations in the resistance was confirmed by genetic complementation. Blocking of virus entry into the mutants was demonstrated by the lack of early gene transcription, strongly supporting the idea of a role of the proteins in SIRV2 entry.