Purification and characterization of antimicrobial peptides from the skin secretions of the carpenter frog Rana virgatipes (Ranidae, Aquarana)
Purification and characterization of antimicrobial peptides from the skin secretions of the carpenter frog Rana virgatipes (Ranidae, Aquarana)
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DOI：
10.1016/j.regpep.2005.06.003
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发表时间：
2005-11-15
影响因子：
--
通讯作者：
Bevier, CR
中科院分区：
文献类型：
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作者：
Conlon, JM;Abraham, B;Bevier, CR
Aquarana（或Rana catesbeiana物种组）的成员形成了一个得到充分支持的单系分支，但组内物种之间的系统发育关系尚不完全清楚。差异抑制细菌生长的肽纯化从电刺激的皮肤分泌物的木匠蛙virgatipes。结构表征鉴定了先前在红毛鼠皮肤中发现的ranatuerin-2（3肽）和temporin（3肽）家族的成员。卡特斯贝亚纳河克拉米坦斯河grylio和R. septentrionalis。Ranalexin是一种以前只在Aquarana中发现的肽，与一种变体（FFGLHNLVPSMLCVVRKKC）一起分离，该变体缺乏采用α-螺旋构象的倾向，因此缺乏抗菌活性。从皮肤分泌物中分离到两个属于brevinin-2家族的C-末端α-酰胺化肽，它们与R. septentrionalis缺乏与该家族成员相关的C-末端环状七肽结构域。Ranatuerin-1，以前从R.卡特斯贝亚纳河clamitans和R. grylio但缺席R. septentrionalis，在R. virgatipes。temporin-1Va（FLSSIGKILGNLL.NH2）、temporin-1Vb（FLSIIAKVLGSLF.NH2）和temporin-1Vc（FLPLVTMLLGKLF.NH2）的合成复制品有效抑制革兰氏阳性菌（包括耐甲氧西林金黄色葡萄球菌）的生长。Temporin-1Va对革兰氏阴性菌和机会性酵母病原体白色念珠菌也有活性，并且具有相对弱的溶血活性（LD 50 =120 μ M），因此可能代表药物开发的候选者。我们的数据支持R. virgatipes在Aquarana中的亲缘关系较近，表明R. virgatipes与R. septentrionalis比R.卡特斯贝亚纳河clamitans和R. grylio。(c)2005年由Elsevier B. V.出版
The members of the Aquarana (or Rana catesbeiana species group) form a well-supported monophyletic clade but phylogenetic relationships between species within the group are incompletely understood. Peptides that differentially inhibited the growth of bacteria were purified from electrically stimulated skin secretions of the carpenter frog Rana virgatipes. Structural characterization identified members of the ranatuerin-2 (3 peptides) and temporin (3-peptides) families, previously found in the skins of R. catesbeiana, R. clamitans, R. grylio and R. septentrionalis. Ranalexin, a peptide previously found only in the Aquarana, was isolated together with a variant (FFGLHNLVPSMLCVVRKKC) that lacks the propensity to adopt an alpha-helical conformation and so was devoid of antimicrobial activity. Two C-terminally a-amidated peptides belonging to the brevinin-2 family were isolated from the skin secretions that, like an ortholog from R. septentrionalis, lacked the C-terminal cyclic heptapeptide domain associated with members of this family. Ranatuerin-1, previously isolated from R. catesbeiana, R. clamitans and R. grylio but absent from R. septentrionalis, was also not identified in R. virgatipes. Synthetic replicates of temporin-1Va (FLSSIGKILGNLL.NH2), temporin-1Vb (FLSIIAKVLGSLF.NH2) and temporin-1Vc (FLPLVTMLLGKLF.NH2) potently inhibited growth of Gram-positive bacteria (including methicillin-resistant Staphylococcus aureus). Temporin-1Va was also active against Gram-negative bacteria and the opportunistic yeast pathogen Candida albicans and had relatively weak hemolytic activity (LD50=120 mu M) and may therefore represent a candidate for drug development. Our data support the placement of R. virgatipes in the Aquarana and indicate a closer phylogenetic relationship of R. virgatipes with R. septentrionalis than with R. catesbeiana, R. clamitans and R. grylio. (c) 2005 Published by Elsevier B.V.