Enterococcus faecalis Readily Adapts Membrane Phospholipid Composition to Environmental and Genetic Perturbation.
Enterococcus faecalis Readily Adapts Membrane Phospholipid Composition to Environmental and Genetic Perturbation.
复制标题
DOI：
10.3389/fmicb.2021.616045
复制
发表时间：
2021
影响因子：
5.2
通讯作者：
Fozo EM
中科院分区：
文献类型：
--
作者：
Woodall BM;Harp JR;Brewer WT;Tague ED;Campagna SR;Fozo EM
由脂肪酸（酰基）尾部和极性头部基团组成的细菌脂质膜通过改变酰基尾部和/或头部基团来响应变化的条件。这种可塑性对于细胞存活至关重要，因为它允许维持膜的保护性质以及功能性膜蛋白复合物。生活在富含脂肪酸的环境中的细菌，例如在人类宿主中发现的那些，可以利用宿主脂肪酸来合成它们自己的膜，从而改变它们的生理机能。粪肠球菌就是这样一种生物：它是哺乳动物肠道的共生体，暴露于富含脂肪酸的胆汁中，也是医院感染的主要原因，在此期间它暴露于含脂肪酸的血清。我们采用了一种非靶向的方法来检测大肠杆菌中最常见的磷脂种类。通过超高效液相色谱高分辨率质谱法（UHPLC-HRMS）测定粪肠球菌OG 1 RF。我们不仅研究了组合物在暴露于宿主脂肪酸时如何响应，而且还研究了预测合成主要极性头部基团的基因的缺失如何影响脂质组合物。无论遗传背景和不同的基础脂质组成，所有菌株都能够改变其脂质组成后，暴露于个别宿主脂肪酸。然而，特定的基因缺失菌株改变了对膜损伤剂的存活。结合起来，肠球菌脂质组对遗传和环境扰动的反应具有高度弹性，可能有助于应激生存。
The bacterial lipid membrane, consisting both of fatty acid (acyl) tails and polar head groups, responds to changing conditions through alteration of either the acyl tails and/or head groups. This plasticity is critical for cell survival as it allows maintenance of both the protective nature of the membrane as well as functioning membrane protein complexes. Bacteria that live in fatty-acid rich environments, such as those found in the human host, can exploit host fatty acids to synthesize their own membranes, in turn, altering their physiology. Enterococcus faecalis is such an organism: it is a commensal of the mammalian intestine where it is exposed to fatty-acid rich bile, as well as a major cause of hospital infections during which it is exposed to fatty acid containing-serum. Within, we employed an untargeted approach to detect the most common phospholipid species of E. faecalis OG1RF via ultra-high performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). We examined not only how the composition responds upon exposure to host fatty acids but also how deletion of genes predicted to synthesize major polar head groups impact lipid composition. Regardless of genetic background and differing basal lipid composition, all strains were able to alter their lipid composition upon exposure to individual host fatty acids. Specific gene deletion strains, however, had altered survival to membrane damaging agents. Combined, the enterococcal lipidome is highly resilient in response to both genetic and environmental perturbation, likely contributing to stress survival.
登录
查看更多内容
影响因子：
3.7
作者：
Mishra NN;Bayer AS;Tran TT;Shamoo Y;Mileykovskaya E;Dowhan W;Guan Z;Arias CA
通讯作者：
Arias CA
影响因子：
5.2
作者：
Bayer AS;Schneider T;Sahl HG
通讯作者：
Sahl HG
影响因子：
4.8
作者：
Cuypers, Maxime G.;Subramanian, Chitra;Rock, Charles O.
通讯作者：
Rock, Charles O.
影响因子：
4.4
作者：
Brewer W;Harrison J;Saito HE;Fozo EM
通讯作者：
Fozo EM
影响因子：
3
作者：
Garcia Montes de Oca, Led Yered Jafet;Cabellos Avelar, Tecilli;Berta Gutierrez-Cirlos, Emma
通讯作者：
Berta Gutierrez-Cirlos, Emma