Mechanical and biochemical properties of human cervical tissue
Mechanical and biochemical properties of human cervical tissue
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DOI：
10.1016/j.actbio.2007.04.009
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发表时间：
2008-01-01
影响因子：
9.7
通讯作者：
Socrate, S.
中科院分区：
文献类型：
--
作者：
Myers, K. M.;Paskaleva, A. P.;Socrate, S.
宫颈组织的机械完整性对于维持健康妊娠至关重要。组织生物化学的改变会导致宫颈机械特性发生巨大变化，并导致宫颈过早扩张和分娩。我们对人类子宫切除标本的宫颈样本的机械和生化特性进行了研究。研究了三个临床病例：既往阴道分娩的非妊娠子宫切除患者；既往没有阴道分娩史的非妊娠子宫切除患者；以及剖宫产时子宫切除的妊娠患者。组织样本在受限压缩、非受限压缩和张力下进行测试。还对三个临床病例的宫颈组织样本进行了生化分析。生化测定测量宫颈组织水合、胶原蛋白含量、胶原蛋白可提取性和硫酸化糖胺聚糖 (GAG) 含量。机械测试结果表明，宫颈基质具有非线性、时间依赖性应力反应，根据其产科背景，具有不同程度的调节和滞后。结果发现，非妊娠组织在拉伸和压缩方面均明显比妊娠组织硬。此外，妊娠组织中的胶原蛋白提取率、硫酸化 GAG 含量和水合作用显着更高。这项研究是加深对宫颈组织分子结构与其宏观力学性能之间复杂相互作用的理解的重要第一步。 (C) 2007 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
The mechanical integrity of cervical tissue is crucial for maintaining a healthy gestation. Altered tissue biochemistry can cause drastic changes in the mechanical properties of the cervix and contribute to premature cervical dilation and delivery. We present an investigation of the mechanical and biochemical properties of cervical samples from human hysterectomy specimens. Three clinical cases were investigated: nonpregnant hysterectomy patients with previous vaginal deliveries; nonpregnant hysterectomy patients with no previous vaginal deliveries; and pregnant hysterectomy patients at time of cesarean section. Tissue samples were tested in confined compression, unconfined compression and tension. Cervical tissue samples for the three clinical cases were also subjected to biochemical analysis. Biochemical assays measured cervical tissue hydration, collagen content, collagen extractability and sulfated glycosaminoglycan (GAG) content. Results from the mechanical tests indicate that cervical stroma has a nonlinear, time-dependent stress response with varying degrees of conditioning and hysteresis depending on its obstetric background. It was found that the nonpregnant tissue was significantly stiffer than the pregnant tissue in both tension and compression. Further, collagen extractability, sulfated GAG content and hydration were substantially higher in the pregnant tissue. This study is the first important step towards the attainment of an improved understanding of the complex interplay between the molecular structure of cervical tissue and its macroscopic mechanical properties. (C) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.