Protocol to study circadian food-anticipatory poking in mice using the feeding experimentation device version 3.
Protocol to study circadian food-anticipatory poking in mice using the feeding experimentation device version 3.
复制标题
使用喂养实验装置第 3 版研究小鼠昼夜节律食物预期刺激的方案。
DOI：
10.1016/j.xpro.2024.102935
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Yamazaki,Shin
中科院分区：
文献类型：
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作者：
Ehichioya,DavidE;Masud,Ishrat;Taufique,SKTahajjul;Jeong,Byeongha;Farah,Sofia;Eischeid,Averey;Balaji,Khaviya;Shen,Melody;Takahashi,JosephS;Yamazaki,Shin
食物预期性戳鼻是一种独特的食物搜寻行为，由食物敏感振荡器驱动。在这里，我们提出了一个协议，以记录一种新的食物寻找鼻子戳行为的小鼠在时间限制喂养，然后使用开源喂养实验设备版本3（FED 3）的食物剥夺。我们描述了设置FED 3和笼子，训练和习惯化的步骤。然后，我们详细介绍了建立时间限制喂养和食物剥夺时间表的程序，以及从FED 3数据生成行为图的程序。有关本方案使用和执行的完整详细信息，请参见Ehichioya et al.1
Food-anticipatory nose poking is a unique food-seeking behavior driven by the food-entrainable oscillator. Here, we present a protocol to record a novel food-seeking nose poking behavior in mice under temporally restricted feeding followed by food deprivation using the open-source feeding experimentation device version 3 (FED3). We describe steps for setting up the FED3 and cage, training, and habituation. We then detail procedures for setting up the schedule for time-restricted feeding and food deprivation and for generating ethograms from FED3 data.For complete details on the use and execution of this protocol, please refer to Ehichioya et al.1