Echinococcus multilocularis metacestode extract triggers human basophils to release interleukin-4
Echinococcus multilocularis metacestode extract triggers human basophils to release interleukin-4
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DOI：
10.1111/j.0141-9838.2004.00724.x
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发表时间：
2004-10-01
影响因子：
2.2
通讯作者：
Haas, H
中科院分区：
文献类型：
--
作者：
Aumüller, E;Schramm, G;Haas, H
寄生蠕虫感染与辅助性T细胞2（Th 2）免疫应答和IgE产生相关。然而，其基本机制仅得到部分理解。最近，我们已经从曼氏血吸虫卵提取物中分离出一种蛋白质，该蛋白质触发来自非致敏供体的人嗜碱性粒细胞释放白细胞介素-4（IL-4），这是Th 2应答的关键细胞因子。我们称这种蛋白质为IPSE（来自曼氏血吸虫卵的IL-4诱导原理）。假设IPSE样IL-4诱导活性可能是不同蠕虫物种共有的一般原则，我们研究了绦虫E.多房的影响，其对人类嗜碱性粒细胞。结果表明，E.多房性引起嗜碱性粒细胞脱粒，以及组胺、IL-4和IL-13的分泌，呈剂量依赖性。嗜碱性粒细胞的IgE剥离和再敏化表明IL-4诱导的机制需要细胞上存在IgE。由于先前已经证明了IPSE的类似性质，我们认为S。mansoni和E.多房红细胞可以通过相关机制，即通过诱导嗜碱性粒细胞释放IL-4，在其宿主中诱导Th 2应答。
Infections with parasitic helminths are associated with a T helper 2 (Th2) immune response and IgE production. The underlying mechanism, however, is only partially understood. Recently we have isolated a protein from extracts of Schistosoma mansoni eggs that triggers human basophils from non-sensitized donors to release interleukin-4 (IL-4), the key cytokine of a Th2 response. We called this protein IPSE (for IL-4-inducing principle from Schistosoma mansoni eggs). Supposing that IPSE-like IL-4-inducing activities might be a general principle shared among different helminth species, we investigated extracts from the cestode E. multilocularis for its effect on human basophils. Our results showed that extracts from metacestodes of E. multilocularis cause basophil degranulation, as well as the secretion of histamine, IL-4 and IL-13, in a dose-dependent manner. IgE stripping and resensitization of basophils indicated that the mechanism of IL-4 induction requires the presence of IgE on the cells. Since analogous properties have been demonstrated earlier for IPSE, we think that S. mansoni and E. multilocularis may induce a Th2 response in their hosts via a related mechanism, namely, by the induction of IL-4 release from basophils.