Exosomes derived from endoplasmic reticulum-stressed liver cancer cells enhance the expression of cytokines in macrophages via the STAT3 signaling pathway
Exosomes derived from endoplasmic reticulum-stressed liver cancer cells enhance the expression of cytokines in macrophages via the STAT3 signaling pathway
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DOI：
10.3892/ol.2020.11609
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发表时间：
2020-07-01
期刊：
影响因子：
2.9
通讯作者：
Sun, Guoping
中科院分区：
文献类型：
--
作者：
He, Chengqun;Hua, Wei;Sun, Guoping
先前的研究表明内质网应激通过调节常驻免疫细胞在形成免疫抑制微环境中起重要作用。然而，内质网应激的肿瘤细胞与免疫细胞之间的通讯尚不完全清楚。外泌体在细胞间通讯中起着至关重要的作用。因此，为了研究内质网应激相关外泌体在肝癌细胞介导的巨噬细胞功能重塑中的作用，我们进行了免疫组织化学分析、免疫荧光western-blotting和细胞因子头阵列分析。结果表明，葡萄糖调节蛋白78 （GRP78）在人肝癌组织中表达上调。此外，69.09%的grp78阳性肝癌组织具有表达CD68（+）的巨噬细胞(r=0.55
Previous studies have shown that endoplasmic reticulum (ER) stress serves an important role in shaping the immunosuppressive microenvironment by modulating resident immune cells. However, the communication between ER-stressed tumor cells and immune cells is not fully understood. Exosomes have been reported to play a vital role in intercellular communication. Therefore, in order to investigate the role of ER stress-related exosomes in liver cancer cells mediated macrophage function remodeling, immunohistochemical analysis, western-blotting immunofluorescence and cytokine bead array analyses were performed. The results demonstrated that glucose-regulated protein 78 (GRP78) expression was upregulated in human liver cancer tissue. Moreover, 69.09% of GRP78-positive liver cancer tissues possessed macrophages expressing CD68(+)(r=0.55; P