Apoptosis induction by epigallocatechin gallate involves its binding to Fas
Apoptosis induction by epigallocatechin gallate involves its binding to Fas
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DOI：
10.1006/bbrc.2001.5293
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发表时间：
2001-08-03
影响因子：
3.1
通讯作者：
Isemura, M
中科院分区：
文献类型：
--
作者：
Hayakawa, S;Saeki, K;Isemura, M
已知epigallocatechin Gallate（EGCG）在各种类型的肿瘤细胞中诱导凋亡，但是EGCG诱导细胞凋亡的确切机制仍有待阐明。 FAS-FAS配体系统是凋亡级联反应中的主要途径之一。这项研究的目的是检查EGCG结合FA的可能性触发FAS介导的凋亡。 EGCG对人单核细胞性白血病U937细胞的治疗导致caspase 8活性升高和caspase 8的碎片化。caspase 8抑制剂抑制了由EGCG治疗引起的DNA梯子形成。这些发现表明，FAS介导的级联反应参与了U937细胞中EGCG诱导的凋亡。亲和色谱显示EGCG和FAS之间的结合。因此，结果表明，eGCG结合FA的FA（大概在细胞表面）会触发FAS介导的U937细胞中的凋亡。 （c）2001学术出版社。
Epigallocatechin gallate (EGCG) is known to induce apoptosis in various types of tumor cells, but the precise mechanism by which EGCG induces apoptosis remains to be elucidated. The Fas-Fas ligand system is one of the major pathways operating in the apoptotic cascade. The aim of this study was to examine the possibility that EGCG-binding to Fas triggers the Fas-mediated apoptosis. The EGCG treatment of human monocytic leukemia U937 cells resulted in elevation of caspase 8 activity and fragmentation of caspase 8. The DNA ladder formation caused by the EGCG treatment was inhibited by the caspase 8 inhibitor. These findings suggested the involvement of the Fas-mediated cascade in the EGCG-induced apoptosis in U937 cells. Affinity chromatography revealed the binding between EGCG and Fas. Thus, the results suggest that EGCG-binding to Fas, presumably on the cell surface, triggers the Fas-mediated apoptosis in U937 cells. (C) 2001 Academic Press.