THE PATTERN OF MAMMALIAN BRAIN GANGLIOSIDES—III
THE PATTERN OF MAMMALIAN BRAIN GANGLIOSIDES—III
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哺乳动物脑神经节苷脂的模式-III
DOI：
10.1111/j.1471-4159.1965.tb10256.x
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发表时间：
1965
影响因子：
4.7
通讯作者：
Kunihiko Suzuki
中科院分区：
文献类型：
--
作者：
Kunihiko Suzuki
在正常大脑中，神经节苷脂以各种分子种类的混合物存在。在薄层色谱法分离的单个神经节苷脂斑点上直接测定N-乙酰神经氨酸（NANA）的方法已被发现是简单、准确、重现性好的方法，适用于分析大量小样品（Suzuki，1964）。确定了从脑组织中提取神经节苷脂的最佳工艺。初步实验还表明，没有使用福尔马林分析死后大脑神经节苷脂的有效性（铃木，1965 b）。这种分析方法现在已被应用到哺乳动物脑神经节苷脂模式的区域和发育差异的评价。
IN NORMAL brain, gangliosides are present as a mixture of various molecular species. A method of direct N-acetylneuraminic acid (NANA) determination on the spots of individual gangliosides separated by thin-layer chromatography has been found to be simple, accurate, reproducible, and suitable for analysing a large number of small size samples (SUZUKI, 1964). The optimal extraction procedures for gangliosides from brain tissue have been determined with this method. Preliminary experiments also revealed the validity of analysing post-mortem brains for gangliosides providing formalin had not been used (SUZUKI, 1965b). This analytical method has now been applied to the evaluation of the regional and developmental differences in mammalian brain ganglioside patterns.