Protective efficacy of a recombinant herpesvirus of turkeys as an in ovo vaccine against Newcastle and Marek's diseases in specific pathogen-free chickens
Protective efficacy of a recombinant herpesvirus of turkeys as an in ovo vaccine against Newcastle and Marek's diseases in specific pathogen-free chickens
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DOI：
10.1016/0264-410x(95)00242-s
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发表时间：
1996-04-01
期刊：
影响因子：
5.5
通讯作者：
Schwartz, RD
中科院分区：
文献类型：
--
作者：
Reddy, SK;Sharma, JM;Schwartz, RD
我们研究了基于火鸡疱疹病毒(HVT)的重组病毒(RHVT)作为体内疫苗预防新城疫(ND)和马立克氏病(MD)的潜力。表达新城疫病毒Hitchner BI株融合蛋白(F)和血凝素神经氨酸酶(HN)糖蛋白以及新城疫病毒I型病毒(NDV)GA株糖蛋白A和B的rHVT能有效地保护鸡抵抗新城疫和马立克氏病。接种rHVT对雏鸡孵化率和存活率无不良影响。在鸡胚化第18天(ED18)或孵化时一次给药可保护鸡免受强毒株RB-IB和新城疫强毒株GB-Texas(NDV-GB-TX)的攻击。通过血清学试验，即对新城疫病毒的血凝抑制、病毒中和和免疫印迹试验以及对新城疫病毒的免疫荧光和放射免疫沉淀试验，接种疫苗的鸡都产生了针对这两种病毒的抗体。聚合酶链式反应(PCR)分析表明，rHVT免疫后的雏鸡持续感染新城疫，对新城疫的免疫持续时间长达8周，但病毒分离试验表明，rHVT免疫的雏鸡对新城疫病毒在气管内的复制仅有部分保护作用。研究结果表明，rHVT对新城疫和马立克氏病的ED18疫苗和疫苗后接种都是安全的，而且由于疫苗的持久性，它可能比传统的五种新城疫疫苗诱导更长的持久免疫。版权所有(C)1996爱思唯尔科学有限公司。
We investigated the potential of a herpesvirus of turkey (HVT)-based recombinant virus (rHVT) as an in ovo vaccine to protect specific-pathogen-free chickens against Newcastle disease (ND) and Marek's disease (MD). The rHVT, designed to express fusion (F) and hemogglutinin-neuraminidase (HN) glycoproteins of the lentogenic Hitchner BI strain of ND virus (NDV), as well as glycoproteins A and B of the GA strain of serotype I MD virus (NDV) was efficacious in protecting chickens against ND and MD. No adverse effects on hatchability or the survival of chickens were observed following in ovo vaccination with rHVT. A single administration at embryonation day 18 (ED18) or at hatch protected chickens against challenge-exposures with virulent MDV strain RB-IB and velogenic NDV strain GB-Texas (NDV-GB-TX). Vaccinated chickens developed antibodies against both viruses as detected by serological tests, namely, hemagglutination inhibition, virus neutralization and western immunoblotting for NDV and immunofluorescence and radioimmunoprecipitation assays for MDV. PCR analysis showed that in ovo vaccination with rHVT resulted in a persistent infection leading to systemic immunity against ND for up to 8 weeks of age, the longest period of time tested in this study, However, virus isolation tests indicated that rHVT-vaccinated chickens were only partially protected from the replication of NDV-GB-TX in the trachea. The results of the study indicate that rHVT is safe for both ED18 and posthatch vaccination for ND and MD, and because the vaccine persists, it may induce longer lasting immunity than conventional five NDV vaccines. Copyright (C) 1996 Elsevier Science Ltd.