Test-retest reproducibility of [11C]PBR28 binding to TSPO in healthy control subjects
Test-retest reproducibility of [11C]PBR28 binding to TSPO in healthy control subjects
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DOI：
10.1007/s00259-015-3149-8
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发表时间：
2016-01-01
影响因子：
9.1
通讯作者：
Cervenka, S.
中科院分区：
文献类型：
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作者：
Collste, K.;Forsberg, A.;Cervenka, S.
PET放射配体[C-11]PBR28与转运蛋白（TSPO）结合，TSPO是脑免疫激活的标志。我们以区域和逐体素为基础进行定量，检验了健康受试者中[C-11]PBR28结合的可重复性。此外，我们对TSPO可用性的日变化进行了初步分析。方法采用高分辨率研究层析成像仪和[C-11]PBR28对12名受试者进行检查，其中6名在同一天的上午和下午进行检查，6名在两天的上午进行检查。区域分布体积（V-T）是使用基于兴趣区域的两组织区室分析（2TCM）以及参数方法得出的。经代谢校正的动脉血浆作为输入函数。结果在整个样本中，灰质（GM） V (T)的平均绝对变异性为18.3±12.7%。转基因区类内相关系数为0.90 ~ 0.94。将分析时间从91分钟减少到63分钟，变异率为16.9 +/- 14.9%。参数与2tcm衍生的GM值之间有很强的相关性（r=0.99）。使用二级定量方法时，在上午和下午检查期间观察到GM V-T显著增加（p=0.028）。在当天同一时间检查的受试者中，对于91 min 2TCM数据，绝对变异性为15.9 +/- 12.2%。结论[C-11]PBR28结合的V-T在GM区域具有中等重复性和高可靠性。我们的研究结果支持使用参数方法来确定[C-11]PBR28 V-T值，并表明采集时间可以缩短。脑内TSPO结合的日变化可能是临床研究中一个潜在的混杂因素，应该进一步研究。
Purpose The PET radioligand [C-11]PBR28 binds to the translocator protein (TSPO), a marker of brain immune activation. We examined the reproducibility of [C-11]PBR28 binding in healthy subjects with quantification on a regional and voxel-by-voxel basis. In addition, we performed a preliminary analysis of diurnal changes in TSPO availability.Methods Twelve subjects were examined using a high-resolution research tomograph and [C-11]PBR28, six in the morning and afternoon of the same day, and six in the morning on two separate days. Regional volumes of distribution (V-T) were derived using a region-of-interest based two-tissue compartmental analysis (2TCM), as well as a parametric approach. Metabolite-corrected arterial plasma was used as input function.Results For the whole sample, the mean absolute variability in V (T) in the grey matter (GM) was 18.3 +/- 12.7 %. Intraclass correlation coefficients in GM regions ranged from 0.90 to 0.94. Reducing the time of analysis from 91 to 63 min yielded a variability of 16.9 +/- 14.9 %. There was a strong correlation between the parametric and 2TCM-derived GM values (r=0.99). A significant increase in GM V-T was observed between the morning and afternoon examinations when using secondary methods of quantification (p=0.028). In the subjects examined at the same time of the day, the absolute variability was 15.9 +/- 12.2 % for the 91-min 2TCM data.Conclusion V-T of [C-11]PBR28 binding showed medium reproducibility and high reliability in GM regions. Our findings support the use of parametric approaches for determining [C-11]PBR28 V-T values, and indicate that the acquisition time could be shortened. Diurnal changes in TSPO binding in the brain may be a potential confounder in clinical studies and should be investigated further.