Different Lipopolysaccharide Branched-Chain Amino Acids Modulate Porcine Intestinal Endogenous β-Defensin Expression through the Sirt1/ERK/90RSK Pathway
Different Lipopolysaccharide Branched-Chain Amino Acids Modulate Porcine Intestinal Endogenous β-Defensin Expression through the Sirt1/ERK/90RSK Pathway
复制标题
不同脂多糖支链氨基酸通过 Sirt1/ERK/90RSK 途径调节猪肠内源性 β-防御素表达。
DOI：
10.1021/acs.jafc.6b00968
复制
发表时间：
2016-05-04
影响因子：
6.1
通讯作者：
Qiao, Shiyan
中科院分区：
文献类型：
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作者：
Ren, Man;Zhang, Shihai;Qiao, Shiyan
内源性抗菌肽表达的营养诱导被认为是抑制哺乳动物病原微生物生长和感染的有前途的方法。本研究在体内和体外研究了猪上皮β-防御素响应支链氨基酸（BCAA）的可能调节。 BCAA 处理增加了断奶仔猪空肠和回肠 β-防御素的相对 mRNA 表达。在 IPEC-J2 细胞中，异亮氨酸、亮氨酸和缬氨酸可以刺激 β-防御素表达，可能与刺激 ERK1/2 磷酸化有关。抑制Sirt1和ERK可完全阻断异亮氨酸对ERK和90RSK蛋白的激活，同时降低防御素的表达。支链氨基酸。异亮氨酸最能刺激猪肠上皮 β-防御素的表达，可能通过激活 Sirt1/ERK/90RSK 信号通路发挥作用。脂多糖的β-防御素调节与不依赖于ERK的途径有关。 BCAA 调节内源性防御素可能是增强幼年动物和儿童的抗病能力和肠道健康的一种有前景的方法。
Nutritional induction of endogenous antimicrobial peptide expression is considered a promising approach to inhibit the outgrowth and infection of pathogenic microbes in mammals. The present study investigated possible regulation of porcine epithelial beta-defensins in response to branched-chain amino acids (BCAA) in vivo and in vitro. BCAA treatment increased relative mRNA expression of jejunal and ileal beta-defensins in weaned piglets. In IPEC-J2 cells, isoleucine, leucine, and valine could stimulate beta-defensin expression, possibly associated with stimulation of ERK1/2 phosphorylation. Inhibition of Sirt1 and ERK completely blocked the activation of ERK and 90RSK protein by isoleucine, simultaneously decreasing defensin expression. BCAA. stimulate expression of porcine intestinal epithelial beta-defensins with isoleucine the most, potent possibly through activation of the Sirt1/ERK/90RSK signaling pathway. The beta-defensins regulation of lipopolysaccharide was related with an ERK-independent pathway. BCAA modulation of endogenous defensin might be a promising approach to enhance disease resistance and intestinal health in young animals and children.