Expression of cellular protooncogenes in the mouse male germ line: a distinctive 2.4-kilobase pim-1 transcript is expressed in haploid postmeiotic cells.
Expression of cellular protooncogenes in the mouse male germ line: a distinctive 2.4-kilobase pim-1 transcript is expressed in haploid postmeiotic cells.
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小鼠雄性种系中细胞原癌基因的表达：独特的 2.4 KB pim-1 转录物在单倍体减数分裂后细胞中表达。
DOI：
10.1073/pnas.85.7.2191
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发表时间：
1988
影响因子：
11.1
通讯作者：
Fleissner,E
中科院分区：
文献类型：
--
作者：
Sorrentino,V;McKinney,MD;Giorgi,M;Geremia,R;Fleissner,E
我们报道了2.4kb的Pim-1转录本在小鼠睾丸的生殖细胞中表达。对精细胞类型的纯化群体分析表明，2.4-kb转录本选择性地在单倍体减数分裂后的早期精子细胞中表达。Pim-1在单倍体精子细胞中表达受发育调控的证据表明，这种原癌基因产物可能在发育中发挥作用。减数分裂后细胞中存在的2.4kb的Pim-1转录本与通常在体细胞组织中检测到的2.8kb的转录本在大小上不同。类似的睾丸特异转录本也被发现存在于mos和abl基因中。这些数据表明，在单倍体雄性生殖细胞中，某些基因的转录或处理具有特异性。我们还分析了其他具有代表性的原癌基因，包括蛋白激酶、ras家族和“核”原癌基因的例子。结果表明，其他原癌基因在减数分裂粗线期细胞或减数分裂后早期精子细胞中优先表达。这些发现表明，生殖细胞的这两个发育阶段的基因表达存在差异调节。特别是，对ras基因家族三个成员的表达分析表明，Harvey、Kirsten和N-ras基因在这些生殖细胞中的表达存在明显的时间差异调节。
We report that a 2.4-kilobase (kb) pim-1 transcript is expressed in the germ cells of mouse testis. Analysis of purified populations of spermatogenic cell types indicates that the 2.4-kb transcript is selectively expressed in haploid postmeiotic early spermatids. The evidence for a developmentally regulated expression of pim-1 in haploid spermatids suggests a possible developmental role for this protooncogene product. The 2.4-kb pim-1 transcript present in postmeiotic cells differs in size from the 2.8-kb transcript usually detected in somatic tissues. Similar testis-specific transcripts have been seen for mos and abl genes. These data suggest specificity in transcription or processing of certain genes in haploid male germ cells. We have also analyzed other representative protooncogenes, including examples of protein kinases, the ras family, and the "nuclear" protooncogenes. The results indicate that additional protooncogenes are preferentially expressed in either meiotic pachytene cells or postmeiotic early spermatids. These findings suggest a differential regulation of gene expression in these two developmental stages of germ cells. In particular, analysis of expression of the three members of the ras gene family indicates a distinct temporal differential regulation in the expression of the Harvey, Kirsten, and N-ras genes in these germ cells.
六种小鼠 α-微管蛋白 mRNA 编码五种不同的同种型：两个姐妹基因的睾丸特异性表达
影响因子：
5.3
作者：
A. Villasanté;D. Wang;P. Dobner;P. Dolph;S. Lewis;N. Cowan
通讯作者：
N. Cowan