Ligand-dependent release of active neutrophil collagenase.
Ligand-dependent release of active neutrophil collagenase.
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活性中性粒细胞胶原酶的配体依赖性释放。
DOI：
10.1002/art.1780330211
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发表时间：
1990
影响因子：
--
通讯作者：
BlackburnJr,WD
中科院分区：
文献类型：
--
作者：
Chatham,WW;Heck,LW;BlackburnJr,WD
活性中性粒细胞（PMN）胶原酶在细胞外的释放量取决于活化PMN的配体。中性粒细胞受可溶性配体（包括FMLP、血小板活化因子或热聚集IgG）刺激时，释放的活性胶原酶很少，与受活化酶酶或表面结合IgG刺激的细胞相比。然而，在影响PMN超氧化物的产生、特定颗粒成分乳铁蛋白的释放或总（潜伏加活性）胶原酶释放方面，活化酶和表面结合的IgG与可溶性配体没有明显的不同，这表明表面结合配体刺激PMN时，胶原酶的激活更有效。这些结果表明，表面（软骨）结合的IgG在类风湿关节炎患者关节中人中性粒细胞胶原酶的释放和激活中起作用。
The amount of active neutrophil (PMN) collagenase released extracellularly is dependent on the PMN‐activating ligand. Neutrophils stimulated with soluble ligands, including FMLP, platelet‐activating factor, or heat‐aggregated IgG, released very little active collagenase, in contrast to cells stimulated with opsonized zymosan or surface‐bound IgG. However, opsonized zymosan and surface‐bound IgG did not differ appreciably from soluble ligands in effecting PMN production of superoxide, release of the specific granule component lactoferrin, or total (latent plus active) collagenase release, which suggests that there is more efficient collagenase activation during PMN stimulation with surface‐bound ligands. These results suggest a role for surface (cartilage)‐bound IgG in the release and activation of human neutrophil collagenase in the joints of patients with rheumatoid arthritis.