Correlative tomography.
Correlative tomography.
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DOI：
10.1038/srep04711
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发表时间：
2014-04-16
影响因子：
4.6
通讯作者：
Withers PJ
中科院分区：
文献类型：
--
作者：
Burnett TL;McDonald SA;Gholinia A;Geurts R;Janus M;Slater T;Haigh SJ;Ornek C;Almuaili F;Engelberg DL;Thompson GE;Withers PJ
越来越多的研究人员希望将多个尺度的观点结合起来，或者结合不同技术的见解，以研究同一感兴趣的区域。为此，关联显微镜已经在二维 (2D) 领域取得了实质性的新见解。在这里，我们开发了相关断层扫描，其中相关任务更具挑战性，因为感兴趣的体积通常隐藏在样本表面下方。我们将 X 射线计算机断层扫描、连续切片 FIB-SEM 断层扫描、电子背散射衍射以及最后的 TEM 元素分析结合在一起，所有这些都针对同一 3D 区域。这使得能够在多个尺度上观察点蚀和晶间腐蚀之间的竞争，揭示不锈钢中隐蔽腐蚀坑的结构层次、晶体学和化学性质。通过自动化关联工作流程和多尺度或多模式数据集的联合可视化，该技术有望提供跨生物、地质和材料科学的见解，而使用单个或多个不相关的技术是不可能实现的。
Increasingly researchers are looking to bring together perspectives across multiple scales, or to combine insights from different techniques, for the same region of interest. To this end, correlative microscopy has already yielded substantial new insights in two dimensions (2D). Here we develop correlative tomography where the correlative task is somewhat more challenging because the volume of interest is typically hidden beneath the sample surface. We have threaded together x-ray computed tomography, serial section FIB-SEM tomography, electron backscatter diffraction and finally TEM elemental analysis all for the same 3D region. This has allowed observation of the competition between pitting corrosion and intergranular corrosion at multiple scales revealing the structural hierarchy, crystallography and chemistry of veiled corrosion pits in stainless steel. With automated correlative workflows and co-visualization of the multi-scale or multi-modal datasets the technique promises to provide insights across biological, geological and materials science that are impossible using either individual or multiple uncorrelated techniques.