SUPERANTIGENS INTERACT WITH MHC CLASS-II MOLECULES OUTSIDE OF THE ANTIGEN GROOVE
SUPERANTIGENS INTERACT WITH MHC CLASS-II MOLECULES OUTSIDE OF THE ANTIGEN GROOVE
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DOI：
10.1016/0092-8674(90)90388-u
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发表时间：
1990-09-21
期刊：
影响因子：
64.5
通讯作者：
MATHIS, D
中科院分区：
文献类型：
--
作者：
DELLABONA, P;PECCOUD, J;MATHIS, D
超抗原，包括葡萄球菌肠毒素和少量淋巴细胞刺激抗原，是一种高效的免疫刺激分子，能够激活几乎所有表达特定T细胞受体(TCR)可变区的T细胞。超抗原对T淋巴细胞的刺激依赖于主要组织相容性复合体(MHC)的II类分子，因此超抗原是否像传统的多肽抗原一样与II类复合体上的抗原结合沟相互作用，或者它们是否与其他地方结合并作为TCR结合，一直存在争议。我们比较了一组呈现突变的细胞系呈现多肽抗原和超抗原的情况，每个突变呈现细胞系都显示一个α-k链，沿着α-链有一个丙氨酸替代。螺旋线建议形成凹槽的一个面。这30个突变对超抗原呈递的影响可以忽略不计，与它们对肽呈递的严重后果相比，促使我们得出结论，超抗原剂与沟槽外的MHC II类分子相互作用。
Superantigens, including the staphyloccal enterotoxins and the minor lymphocyte stimulatory antigens, are highly potent immunostimulatory molecules, capable of activating virtually all T cells that express particular T cell receptor (TCR) variable regions. Superantigen stimulation of T lymphocytes depends on major histocompatibility complex (MHC) class II molecules, so there has been some debate as to whether superantigens interact with the antigen binding "groove" on class II complexes, just like conventional peptide antigens, or whether they bind elsewhere and serve as TCR coligands. We compared the presentation of peptide antigens and superantigens by a panel of mutant-presenting cell lines, each displaying a A.alpha.k chain with a single alanine replacement along the .alpha. helix proposed to form one face of the groove. The negligible effect of these 30 mutations on superantigen presentation, versus their drastic consequences for peptide presentation, prompts us to conclude that superantigents interact with MHC class II molecules outside the groove.