Quantification of expression of netrins, slits and their receptors in human prostate tumors
Quantification of expression of netrins, slits and their receptors in human prostate tumors
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DOI：
10.1002/ijc.10821
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发表时间：
2003-01-20
影响因子：
6.4
通讯作者：
Cussenot, O
中科院分区：
文献类型：
--
作者：
Latil, A;Chêne, L;Cussenot, O
最近，DCC （Deleted in Colorectal Cancer）蛋白被认为是netrin的受体。Netrin/DCC复合物对轴突引导和细胞迁移至关重要。在发育中的神经系统中，中线细胞分泌的netrin蛋白通过激活生长锥上的DCC受体来吸引交轴突。这种吸引可以转换为排斥或完全沉默，这取决于DCC结合伙伴。DCC在前列腺肿瘤发生中的潜在抑制功能，其机制尚不清楚，这促使我们量化了参与这一轴突引导通路的几个基因的表达。采用实时定量逆转录聚合酶链反应（RT-PCR）技术，同时测定了48例肿瘤和7例正常前列腺组织中DCC、NEO1、NTN1、NTN2L、NTN4、UNC5C、Slit1、Slit2、Slit3、Robo1和Robo2的相对表达水平，发现前列腺肿瘤中DCC、NEO1、NTN1和NTN4的表达减少，而许多相同的前列腺肿瘤要么过表达Slit基因，要么过表达其受体Robo。(C) 2002 Wiley-Liss, Inc。
Recently, DCC (Deleted in Colorectal Cancer) protein has been forwarded as a receptor for netrin. The Netrin/DCC complex is critical for axon guidance and cell migration. In the developing nervous system, netrin protein secreted by midline cells attracts commissural axons by activating the DCC receptor on growth cones. This attraction can be switched to repulsion or silenced completely, depending on the DCC binding partner. The potential suppressor function of DCC in prostate tumorigenesis, through a still unknown mechanism, prompted us to quantify the expression of several genes involved in this axon guidance pathway. The relative expression levels of DCC, NEO1, NTN1, NTN2L, NTN4, UNC5C, Slit1, Slit2, Slit3, Robo1 and Robo2 were simultaneous quantified in 48 tumors and 7 normal prostate tissues by using real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), A reduction in DCC, NEO1, NTN1 and NTN4 expression was observed in prostate tumors, while many of the same prostate tumors over-expressed either Slit genes or their receptors, Robo. (C) 2002 Wiley-Liss, Inc.