IMMUNOHISTOCHEMICAL STUDY OF THE DEVELOPMENT OF SEROTONERGIC NEURONS IN THE RAT CNS1
IMMUNOHISTOCHEMICAL STUDY OF THE DEVELOPMENT OF SEROTONERGIC NEURONS IN THE RAT CNS1
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DOI：
10.1016/0361-9230(82)90164-2
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发表时间：
1982-01-01
影响因子：
3.8
通讯作者：
MOLLIVER, ME
中科院分区：
文献类型：
--
作者：
LIDOV, HGW;MOLLIVER, ME
血清素能（5-羟色胺）[5-羟色胺]神经元的发育是从它们的递质合成开始直到基本成熟形态的建立。5-HT免疫细胞化学用于可视化这一过程的精确特征。这种方法的高度稳定性和反染色组织切片的可行性允许树突突和轴突终末以及核周的可视化，并促进了这些结构相对于神经细胞的非5- ht成分的定位。对大鼠胚胎第13、14、15、17、19和21天以及产后第1、3、4和10天的连续横切面和矢状面切片进行了检测。详细的显微图显示了5-HT神经元在所有产前阶段的位置。5-HT神经元的发育是根据它们的细胞形态，特别是树突结构，这些细胞与周围脑干发育的关系以及5-HT核的形态和堆积密度来评估的。基于这些考虑，提出了细胞核内细胞迁移模式的模型，该模式导致5-HT投射上升。E14可见相对简单的双侧上（吻侧）和下（尾侧）5-HT细胞群。在E14-E19期间，这些细胞的几个亚组发展起来，可能是细胞迁移差异的结果。根据这些细胞的主要树突方向，可以重建它们可能的迁移路径。在E19时，5-HT神经元呈组状分布，与成人相似。从E19到出生后第1周结束，5-HT树突生长迅速，细胞堆积密度明显下降。这些变化使核聚集体形成成人的形态。在脑干中缝和内侧网状核的神经发生、迁移和轴突投射的已知特征的背景下，讨论了5-HT细胞群的发育。5-HT核内细胞堆积密度的降低可能反映了中缝核中非5-HT成分的出现。
The development of serotonergic (5-HT) [5-hydroxytryptamine] neurons is followed from their initiation of transmitter synthesis until the establishment of an essentially mature morphology. 5-HT immunocytochemistry was used to visualize the precise features of this process. The great stability of this method and the feasibility of counter-staining tissue sections permit the visualization of dendritic processes and axon terminals, as well as perikarya, and facilitates the localization of these structures with respect to non-5-HT components of the neuropil. Serial transverse and sagittal sections of rat fetuses on embryonic days (E) 13, 14, 15, 17, 19 and 21, and postnatal rats on days 1, 3, 4 and 10 were examined. A detailed photomicrographic map showing the locations of 5-HT neurons at all prenatal stages is provided. The development of 5-HT neurons is evaluated in terms of their cellular morphology, particularly dendritic architecture, the relationship of these cells to the development of the surrounding brainstem and the morphology and packing density of the 5-HT nuclei. From these considerations a model is proposed of the pattern of cell migration within the nuclei that give rise to the ascending 5-HT projections. At E14 a relatively simple configuration of bilateral superior (rostral) and inferior (caudal) 5-HT cell groups is present. In the period extending from E14-E19 several subgroupings of these cells develop, presumably as the result of differential cell migration. Based on the predominant dendritic orientation of these cells it is possible to reconstruct their probable migratory paths. At E19 the 5-HT neurons are distributed in groups that are similar to those seen in the adult. In the time from E19 until the end of the 1st postnatal week, there is rapid growth of 5-HT dendrites and a marked decrease in cellular packing density. These alterations shape the nuclear aggregates into the form seen in the adult. The development of the 5-HT cell groups is discussed in the context of known features of neurogenesis, migration and axonal projections of the raphe and medial reticular nuclei of the brainstem. The possibility is raised that the decrease in cellular packing density in the 5-HT nuclei may reflect the appearance of the non-5-HT components of the raphe nuclei.