A shotgun proteomic method for the identification of membrane-embedded proteins and peptides
A shotgun proteomic method for the identification of membrane-embedded proteins and peptides
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DOI：
10.1021/pr700795f
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发表时间：
2008-07-01
影响因子：
4.4
通讯作者：
Wu, Christine C.
中科院分区：
文献类型：
--
作者：
Blackler, Adele R.;Speers, Anna E.;Wu, Christine C.
整合膜蛋白执行关键的细胞功能，并且是大多数药物制剂的作用靶点。然而，其膜嵌入结构域的疏水性质使得它们难以研究。在此，我们描述了一种鸟枪法蛋白质组学方法，用于对整合膜蛋白的膜嵌入跨膜结构域进行高通量分析，该方法扩大了膜蛋白质组的覆盖深度。
Integral membrane proteins perform crucial cellular functions and are the targets for the majority of pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains makes them difficult to work with. Here, we describe a shotgun proteomic method for the high-throughput analysis of the membrane-embedded transmembrane domains of integral membrane proteins which extends the depth of coverage of the membrane proteome.