The role of microvasculature in normal and perturbed bone healing as revealed by intravital microscopy
The role of microvasculature in normal and perturbed bone healing as revealed by intravital microscopy
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DOI：
10.1016/s8756-3282(96)00133-0
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发表时间：
1996-07-01
期刊：
影响因子：
4.1
通讯作者：
Winet, H
中科院分区：
文献类型：
--
作者：
Winet, H
骨腔活体显微镜将体外模型的控制体积和体内模型的化学复杂性结合起来，用于骨循环的研究。作为一种光学工具，它提供了一个窗口，循环事件在组织水平的放大。特别地，它允许1）通过在任何时刻放大局部灌注的脉管系统和微循环来在空间上测量微血管生理学，以及2）通过提供相同体积的组织来在时间上测量微血管生理学，用于每周观察诸如骨愈合的演变过程。该空心螺钉的窗口揭示了：1）愈合期间血管和骨进展的一致顺序，2）对植入聚合物的反应的血管变化和3）关于高压氧和脉冲电磁场对血管愈合方面的影响的初步数据。测量的参数是骨生成、血管生成、血液供应和渗透性。
Bone chamber intravital microscopy brings to the study of bone circulation a combination of the control volume of in vitro models and the chemical complexity of in vivo models. As an optical tool it provides a window to circulatory events at the tissue level of magnification. In particular, it allows measures of microvascular physiology 1) in space by magnifying local perfused vasculature and microcirculation at any instant and 2) in time by providing the same volume of tissue for weekly viewing of an evolving process such as bone healing. This hollow screw's windows have revealed: 1) a consistent order for vascular and bone progression during healing, 2) vascular changes in response to implanted polymers and 3) preliminary data about effects of hyperbaric oxygenation and pulsed electromagnetic fields on vascular aspects of healing. The parameters measured are osteogenesis, angiogenesis, blood supply and permeability.