Identification of active chemical compounds and potential antibacterial snail mucus (Achatina fulica) on bacteria Enterococcus foecalis causes of periodontitis
Identification of active chemical compounds and potential antibacterial snail mucus (Achatina fulica) on bacteria Enterococcus foecalis causes of periodontitis
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鉴定活性化合物和潜在抗菌蜗牛粘液（Achatina fulica）对引起牙周炎的粪肠球菌细菌的作用
DOI：
10.15562/ijbs.v16i2.426
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发表时间：
2022
影响因子：
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通讯作者：
Regina Tedjasulaksana
中科院分区：
文献类型：
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作者：
I. G. A. Swastini;Ni Nengah Sumerti;Ni Ketut Ramini;Wayan Juniarsana;I. K. Suardana;I. A. M. S. Arjani;Ida Ayu Dewi Kumala Ratih;Regina Tedjasulaksana
背景：化合物是存在于天然来源中的化合物，赋予其植物或动物来源的特殊性质和特征。蜗牛粘液是来自动物的传统药物之一，被社区用作治疗小伤口和牙痛的传统药物。本研究的目的是确定蜗牛粘液中的活性化合物和其对引起牙周炎的粪肠球菌的抗菌潜力。
方法：真实实验实验室研究方法，通过实验室分析蜗牛粘液来获取测试样品中所含的化学元素、化合物的含量。活性化合物的含量基于气相色谱-质谱（GCMS）测试。
结果：四次重复的硫酸乙酰肝素平均含量为16.45 mg/100g，硫酸阿查兰21.33 mg/100g，蛋白质阿查辛102.22 mg/100g，糖蛋白88.6 mg/100g，离子Ca2+ 86.2 mg/100g，β凝集素85.22毫克/100克。毒性试验重复进行四次，浓度分别为100%、50%、25%、12.5%。毒性试验结果最低，100%浓度时值为0.171，而50%浓度时为0.302、25%浓度为0.453、12.5%浓度为0.768，对粪肠球菌的抑制力为23.15mm，属于很强的类别。
结论：蜗牛粘液中的活性化学成分为Acharan硫酸盐、Achasin蛋白、糖蛋白、Ca2+离子、β凝集素，蜗牛粘液对粪肠球菌的抗菌潜力很强。
Background: A chemical compound is a chemical compound present in a natural source that gives it the special properties and characteristics of the plant or animal source. Snail slime is one of the traditional medicines from animals that is used by the community as a traditional medicine for healing minor wounds and toothaches. The aim of this study was to identify the active chemical compounds and antibacterial potential of snail mucus against the bacteria E. foecalis that causes periodontitis.
Method: True experiment laboratory research method which is carried out by laboratory analysis of snail mucus to obtain chemical elements, the content of compounds contained in a test sample. The content of active compounds based on the Gas Chromatography-Mass Spectrometry (GCMS) test.
Results: The average content of heparan sulfate with four repetitions was 16.45 mg/100g, Acharan sulfate 21.33 mg/100g, protein achasin 102.22 mg/100g, Glycoconyugat 88.6 mg/100g, Ion Ca2+ 86.2 mg/100g, Beta agglutinins 85.22 mg/100g. Toxicity test was carried out with four repetitions with concentrations of 100%, 50%, 25%, and 12.5%. And the lowest results of the toxicity test were at a concentration of 100% with a value of 0.171, while at a concentration of 50% 0.302, 25% 0.453, and 12.5% 0.768, for bacteria E. Foecalis with an inhibitory power of 23.15 mm, the category was very strong.
Conclusion: The active chemical content in snail mucus is Acharan sulfate, achasin protein, Glycoconyugat, Ca2+ ion, Beta agglutinin, and the antibacterial potential of snail mucus against E. foecalis bacteria is very strong.