A reversible shearing DNA probe for visualizing mechanically strong receptors in living cells
A reversible shearing DNA probe for visualizing mechanically strong receptors in living cells
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一种可逆剪切 DNA 探针，用于可视化活细胞中机械强度较高的受体
DOI：
10.1038/s41556-021-00691-0
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发表时间：
2021-05-31
影响因子：
21.3
通讯作者：
Liu, Zheng
中科院分区：
文献类型：
--
作者：
Li, Hongyun;Zhang, Chen;Liu, Zheng
在过去的十年中，基于DNA的张力传感器对许多生物系统中机械力的重要性的研究做出了重大贡献。尽管这些技术是成功的，但它们的一个缺点是它们不能可逆地测量更高范围（即>20 pN）的受体力，这限制了我们对活细胞中机械化学转导的分子细节的理解。在这里，我们开发了一种可逆剪切DNA为基础的张力探针（RSDTP）探测分子皮牛顿尺度的力之间的4和60 pN的细胞传输。使用这些探针，我们可以很容易地区分力轴承整合素的差异，而不干扰粘附生物学，并揭示了一个强大的力轴承整合素集群可以作为一个“机械枢轴”，以保持局部粘附结构，并促进其成熟。RSDTP的优点包括高动态范围，可逆性和单分子灵敏度，所有这些都将有助于更好地理解机械生物学的分子机制。
In the last decade, DNA-based tension sensors have made significant contributions to the study of the importance of mechanical forces in many biological systems. Albeit successful, one shortcoming of these techniques is their inability to reversibly measure receptor forces in a higher regime (that is, >20 pN), which limits our understanding of the molecular details of mechanochemical transduction in living cells. Here, we developed a reversible shearing DNA-based tension probe (RSDTP) for probing molecular piconewton-scale forces between 4 and 60 pN transmitted by cells. Using these probes, we can easily distinguish the differences in force-bearing integrins without perturbing adhesion biology and reveal that a strong force-bearing integrin cluster can serve as a ‘mechanical pivot’ to maintain focal adhesion architecture and facilitate its maturation. The benefits of the RSDTP include a high dynamic range, reversibility and single-molecule sensitivity, all of which will facilitate a better understanding of the molecular mechanisms of mechanobiology.