Evaluation of genetic stability and analysis of phytomedicinal potential in micropropagated plants of Rumex nepalensis - A medicinally important source of pharmaceutical biomolecules
Evaluation of genetic stability and analysis of phytomedicinal potential in micropropagated plants of Rumex nepalensis - A medicinally important source of pharmaceutical biomolecules
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DOI：
10.1016/j.jarmap.2017.02.003
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发表时间：
2017-09-01
影响因子：
3.9
通讯作者：
Tandon, Pramod
中科院分区：
文献类型：
--
作者：
Bhattacharyya, Paromik;Kumaria, Suman;Tandon, Pramod
尼泊尔芦笋是印度高海拔地区发现的一种重要的草药。它是各种次生代谢物的丰富来源，在各种草药制剂中广泛使用。对直接茎部器官发生（DSO）和间接茎部器官发生（ISO）两种途径的再生效率进行了实验测试，DSO和ISO途径的再生频率最高，分别为87.2%和91.2%。在添加2mg /l NAA的半强MS培养基中生根率最高。驯化后的植株表型与母株相似。采用起始密码子靶向多态性（SCoT）和随机扩增多态性DNA （RAPD）标记技术对DSO和ISO途径再生植株的克隆稳定性进行了测定。尽管RAPD技术检测到的克隆植株（DSO和ISO）的变异程度较低，但SCoT标记可以检测到相对较高的遗传变异程度，从而证明其优于传统分子标记的有效性。再生方法、植株部位和溶剂体系对各次生代谢产物如酚类、黄酮类、生物碱和单宁含量均有显著影响。同时，抗氧化实验（DPPH和FRAP）对不同组织的抗氧化活性进行了特异性检测，并在iso衍生植物中观察到更高的抗氧化活性值。本报告中记录的更高的繁殖率加上增强的克隆稳定性和次生代谢物的产生，确保了为这种重要药材物种的微繁殖设计的方案的有效性。(C) 2017 Elsevier GmbH版权所有。
Rumex nepalensis is an important medicinal herb found in high altitude regions of India. It is a rich source of various secondary metabolites and has a widespread use in various herbal preparations. The regeneration efficacy of both direct shoot organogenesis (DSO) and indirect shoot organogenesis (ISO) was experimentally tested and the highest regeneration frequency for DSO and ISO pathways were 87.2% and 91.2% respectively. The best rooting frequency was recorded in half-strength MS medium supplemented with 2 mg/l NAA. The plantlets after acclimatization were phenotypically similar to that of the mother plant. The clonal stability of the regenerated plants from both DSO and ISO pathways were determined using Start Codon Targeted Polymorphism (SCoT) and Random amplified polymorphic DNA (RAPD) marker techniques. Although RAPD technique detected lesser degree of variability within the cloned plants (both DSO and ISO), SCoT marker could detect comparatively higher degree of genetic variability thereby proving its efficacy over the conventional molecular markers. Regeneration methodology, plant part and solvent system significantly influenced the levels of various secondary metabolites like phenols, flavonoids, alkaloids and tannins. Simultaneously, the antioxidant assays (DPPH and FRAP) detected the antioxidant activity varied tissue specifically and a significant higher value of antioxidant activity was observed within the ISO-derived plants. Higher multiplication rate coupled with enhanced clonal stability and secondary metabolite production recorded in the present report ensure the efficacy of the protocol designed for the micropropagation of this important medicinal herb species. (C) 2017 Elsevier GmbH. All rights reserved.