To knockout in 129 or in C57BL/6: that is the question
To knockout in 129 or in C57BL/6: that is the question
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DOI：
10.1016/j.tig.2003.12.006
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发表时间：
2004-02-01
影响因子：
11.4
通讯作者：
Burmeister, M
中科院分区：
文献类型：
--
作者：
Seong, E;Saunders, TL;Burmeister, M
传统上，敲除实验在来源于129小鼠品系的ES细胞中进行，然后与更稳健的C57 BL/6品系回交。C57 BL/6衍生的ES细胞仅偶尔用于该过程。我们直接比较了C57 BL/6-和129来源的ES细胞，并复习了文献。我们发现，虽然有些步骤效率较低，但C57 BL/6小鼠的优势足以弥补这些缺点。
Traditionally, knockout experiments are performed in ES cells derived from the 129 mouse strain, followed by backcrossing with the more robust C57BL/6 strain. C57BL/6-derived ES cells have only occasionally been used in this process. We compared C57BL/6- with 129-derived ES cells directly and reviewed the literature. We found that, although some steps are less efficient, the advantages of C57BL/6 mice more than compensate for these drawbacks.