Rift Valley fever virus NSs mRNA is transcribed from an incoming anti-viral-sense S RNA segment
Rift Valley fever virus NSs mRNA is transcribed from an incoming anti-viral-sense S RNA segment
复制标题
DOI：
10.1128/jvi.79.18.12106-12111.2005
复制
发表时间：
2005-09-01
影响因子：
5.4
通讯作者：
Makino, S
中科院分区：
文献类型：
--
作者：
Ikegami, T;Won, S;Makino, S
对纯化的裂谷热病毒（RVFV）颗粒的分析表明存在三个负义RNA基因组，加上三个抗病毒正义RNA片段。病毒体相关的抗病毒正义S片段作为感染后立即合成NSs mRNA的模板。NSs蛋白的合成也发生在感染早期，这表明在感染早期产生的NSs蛋白可能在病毒复制和/或在宿主中的存活中具有生物学意义的作用。用属于布尼亚病毒科的病毒感染的哺乳动物细胞的翻译抑制剂处理通常抑制病毒mRNA合成。然而，RVFV NSs mRNA的合成，而不是N mRNA的合成，在初级转录过程中对嘌呤霉素处理有抗性，这表明RVFV NSs mRNA合成的独特性。
Analysis of purified Rift Valley fever virus (RVFV) particles demonstrated the presence of three negativesense RNA genomes, plus three anti-viral-sense RNA segments. The virion-associated anti-viral-sense S segment served as a template for the synthesis of NSs mRNA immediately after infection. NSs protein synthesis also occurred early in infection, suggesting that NSs protein produced early in infection probably has biologically significant roles in virus replication and/or survival in the host. Translation inhibitor treatment of mammalian cells infected with viruses belonging to the Bunyaviridae family generally inhibits viral mRNA synthesis. However, RVFV NSs mRNA synthesis, but not N mRNA synthesis, was resistant to puromycin treatment during primary transcription, pointing to the uniqueness of RVFV NSs mRNA synthesis.