Transport of organic anions into liver cells and bile.
Transport of organic anions into liver cells and bile.
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将有机阴离子转运至肝细胞和胆汁中。
DOI：
10.1016/0006-2952(80)90165-3
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发表时间：
1980
影响因子：
5.8
通讯作者：
D. M. Woodbury
中科院分区：
文献类型：
--
作者：
D. Rollins;J. Freston;D. M. Woodbury
在稳态条件下，测定了[14C]对乙酰氨基马尿酸([14C]PAAH)和[35S]磺基溴邻苯二甲酸([35S]BSP)在大鼠血浆、肝细胞和胆汁中的分布。测量肝细胞肝窦膜和总胆管内血浆和胆汁之间的电位差。PD为40.3±1.0 mV(Mean±S.E.M.)被记录在窦膜上(细胞相对于胞浆为阴性)。血浆与胆汁之间的PD为3.9±0.5 mV(胆汁相对于血浆为负值)。结合血浆、肝细胞和胆汁中PAAH和BSP的浓度，这些有机阴离子的电势表明这些有机阴离子被积极地通过肝窦膜转运到肝细胞。肝细胞和胆汁中PAAH和BSP的浓度表明，它们是通过主动转运过程通过小管膜转运的。随着血药浓度的增加，PAAH的胆汁/肝细胞比值达到一渐近线，提示PAAH从肝细胞向胆汁的转运是一个载体介导的饱和系统。注入牛磺胆酸钠刺激胆汁流量，增加BSP在胆汁中的排泄，但不影响胆汁中PAH的排泄。PAAH从肝细胞到胆汁的运输似乎起到了“汇”的作用，从而维持了低肝细胞的PAAH浓度。PAAH摄取和排泄的膜转运系统可能与其他外源性和内源性有机阴离子的胆汁排泄有关。这项研究的结果也证明了在研究带电化合物在肝胆系统中的分布时考虑电势的重要性。
The distribution of [14C]para-acetylaminohippuric acid ([14C]PAAH) and [35S]sulfobromophthalein ([35S]BSP) among plasma, liver cells and bile of rats was measured under steady-state conditions. The electrical potential difference (PD) was measured across the sinusoidal membrane of the liver cell and between plasma and bile in the common duct. A PD of 40.3 ± 1.0 mV (mean ± S.E.M.) was recorded across the sinusoidal membrane (cell negative with respect to plasma). The PD between plasma and bile was 3.9 ± 0.5 mV (bile negative with respect to plasma). The electrical potentials, considered together with the concentrations of PAAH and BSP in plasma, liver cells and bile, indicate that these organic anions are actively transported into liver cells across the sinusoidal membrane. The concentrations of PAAH and BSP in liver cells and bile suggest that they are transferred across the canalicular membrane by active transport processes. When the plasma concentration of PAAH was increased, the bile/liver cell ratio of PAAH reached an asymptote, suggesting that the transport of PAAH from liver cells into bile is a carrier-mediated system which can be saturated. An infusion of sodium taurocholate, which stimulated bile flow and increased the excretion of BSP in bile, did not influence the biliary excretion of PAAH. The transport of PAAH from liver cells into bile appears to act as a ‘sink’, thereby maintaining a low liver cell PAAH concentration. The membrane transport system for the uptake and excretion of PAAH may be relevant to the biliary excretion of other exogenous and endogenous organic anions. The results of this study also demonstrate the importance of considering electrical potentials when studying the distribution of a charged compound in the hepato-biliary system.