Evaluation of the cytotoxicity of polyetherurethane (PU) film containing zinc diethyldithiocarbamate (ZDEC) on various cell lines
Evaluation of the cytotoxicity of polyetherurethane (PU) film containing zinc diethyldithiocarbamate (ZDEC) on various cell lines
复制标题
DOI：
10.3349/ymj.2002.43.4.518
复制
发表时间：
2002-08-01
影响因子：
2.4
通讯作者：
Kwon, OH
中科院分区：
文献类型：
--
作者：
Park, JC;Park, BJ;Kwon, OH
含0.1%二乙基二硫代氨基甲酸锌(ZDEC)的聚醚氨酯(PU)膜是测试聚合物基生物材料体外细胞毒性的国际标准物质。目前，直接接触培养L929或BALB/3T3细胞或在提取物稀释条件下培养L929或BALB/3T3细胞是评价生物材料和医疗器械细胞毒性的最常用方法。然而，结果往往是不同的，因为它直接关系到细胞功能和接触细胞的毒性机制。本研究用13个不同组织来源的细胞株检测了含0.1%ZDEC的PU膜(PU-ZDEC)的细胞毒活性。两者之间的相关性。用直接接触法测定各细胞释放的总蛋白，研究反应区的大小和相对细胞毒性。HEPA-1c1c7细胞对反应区大小的敏感性最高，而CHO/DHFR(-)细胞对相对细胞毒性的敏感性最高。在每个细胞系中，未发现两个过程之间的相关性(r=-0.478)。在提取液稀释法中，将细胞培养在不同浓度的ZDEC的培养液中，这些细胞系的细胞毒性随着ZDEC浓度的增加而增加。提取液稀释法对BALB/3T3细胞的敏感性最高。直接接触法和提取液稀释法在各细胞系中的相对细胞毒性比较中未发现相关性(r=-0.445)。在本实验中，13种细胞系中的HEPA-1c1c7、BALB/3T3、CHO/dhfr(-)和L-929细胞是这两种方法的敏感细胞系。初步结果表明，为了进行生物材料的体外细胞毒性测试，有必要对至少一种或多种细胞毒性测试方法和许多细胞系进行比较。
A polyetherurethane (PU) film containing 0.1% zinc diethyldithiocarbamate (ZDEC) is the international standard reference material for testing the in vitro cytotoxicity of polymer based biomaterials. Nowadays, culturing L929 or BALB/3T3 cells in direct contact or in an extract dilution condition is the most frequently using method for evaluating the cytotoxicity from biomaterials and medical devices. However, the results often vary, because it is directly related to the cellular functions and the mechanism of the toxicity of the contacting cells. In this study, 13 cell lines originating from various tissues were used to detect the cytotoxic activities of a PU film containing 0.1% ZDEC (PU-ZDEC). The correlation between. the reactivity zone size and the relative cytotoxicity by quantifying the released total protein from each cell in the direct contact testing method was investigated. Hepa-1c1c7 cells demonstrated the highest sensitivity in the reactivity zone size, while CHO/dhFr(-) cells were the most sensitive in terms of the relative cytotoxicity. A correlation between the two processes in each cell line was not found (r=-0.478). In the extract dilution method, which involved cultivating the cells in the medium with various ZDEC concentrations prepared by diluting the PU incubation, the cytotoxicity increased with increasing ZDEC concentration in all cell lines. The BALB/ 3T3 cells demonstrated the highest sensitivity in the extract dilution method. No correlation in a comparison of the relative cytotoxicity from the direct contact method with the extract dilution method in each cell line, was found (r=-0.445). In this experiment, Hepa-1c1c7, BALB/3T3, CHO/dhFr(-) and L-929 cells among the 13 types of cell lines were the sensitive cell lines according to the two methods. The preliminary results suggest that a comparison of at least one or more cytotoxicity testing methods and many cell lines is necessary for an in vitro cytotoxicity test of biomaterials.