OPDAT1, a plastid envelope protein involved in 12-oxo-phytodienoic acid export for jasmonic acid biosynthesis in Populus
OPDAT1, a plastid envelope protein involved in 12-oxo-phytodienoic acid export for jasmonic acid biosynthesis in Populus
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OPDAT1，一种质体包膜蛋白，参与杨树中茉莉酸生物合成的 12-氧代植物二烯酸输出
DOI：
10.1093/treephys/tpab037
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发表时间：
2021
期刊：
影响因子：
4
通讯作者：
Luo Keming
中科院分区：
文献类型：
--
作者：
Zhao Xin;Li Nannan;Song Qin;Li Xiaohong;Meng Hongjun;Luo Keming
茉莉酸（JA）的环戊烯酮前体-茉莉酮-氧代-植物二烯酸（OPDA）是植物伤害反应所必需的。OPDA是由位于质体中的α-亚麻酸（α-莱亚; 18：3）通过十八烷酸途径合成的，并进一步从质体输出到细胞质中用于JA的生物合成。然而，OPDA从质体的运输机制尚未阐明。在本研究中，质体内定位的蛋白质，命名为12-氧代-苯二烯酸转运蛋白1（OPDAT 1），被鉴定并显示可能参与OPDA从质体输出，在杨。Torr.OPDAT1主要在P.子OPDAT 1在酵母细胞中的功能性表达揭示了OPDAT 1参与OPDA的转运。OPDAT 1基因缺失导致OPDA在杨树质体中的积累增加，JA含量降低，而OPDAT 1基因过量表达的杨树质体中OPDA的积累和JA的合成则相反; OPDAT 1基因在杨树叶片机械损伤后能迅速诱导表达，转基因植株对二斑叶螨（Tetranychus urticae）的抗性增强。这些数据表明OPDAT 1是OPDA的内膜转运蛋白，这对环境胁迫下白杨JA的生物合成具有潜在的意义。
Twelve-oxo-phytodienoic acid (OPDA), the cyclopentenone precursor of jasmonic acid (JA), is required for the wounding response of plants. OPDA is derived from plastid-localized α-linolenic acid (α-LeA; 18:3) via the octadecanoid pathway, and is further exported from plastids to the cytosol for JA biosynthesis. However, the mechanism of OPDA transport from plastids has yet to be elucidated. In the current study, a plastid inner envelope-localized protein, designated 12-oxo-PhtyodienoicAcidTransporter 1 (OPDAT1), was identified and shown to potentially be involved in OPDA export from plastids, inPopulus trichocarpa. Torr.OPDAT1is expressed predominantly in young leaves ofP. trichocarpa. Functional expression ofOPDAT1in yeast cells revealed that OPDAT1 is involved in OPDA transport. Loss-of-function ofOPDAT1in poplar resulted in increased accumulation of OPDA in the extracted plastids and a reduction in JA concentration, whereas anOPDAT1-overexpressing line showed a reverse tendency in OPDA accumulation and JA biosynthesis.OPDAT1transcripts were rapidly induced by mechanical wounding of leaves, and anopdat1mutant transgenic plant displayed increased susceptibility to spider mite (Tetranychus urticae) infestation. Collectively, these data suggest that OPDAT1 is an inner envelope transporter for OPDA, and this has potential implications for JA biosynthesis in poplar under environmental stresses.