Spectrofluorometers are not adequate for aging Aedes and Culex (Diptera: Culicidae) using pteridine fluorescence
Spectrofluorometers are not adequate for aging Aedes and Culex (Diptera: Culicidae) using pteridine fluorescence
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DOI：
10.1603/0022-2585-37.5.769
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发表时间：
2000-09-01
影响因子：
2.1
通讯作者：
Chebret, M
中科院分区：
文献类型：
--
作者：
Lardeux, F;Ung, A;Chebret, M
尝试使用蝶啶荧光水平作为老化个体波利尼西亚伊蚊和致倦库蚊的工具。用技术和装置（即，分光荧光计），可用于发展中国家或野外实验室，并已在其他昆虫群体的类似研究中得出一致的结果。然而，对于Ae。polynesiensis以及Cx.在致倦犬中，个体荧光测量大多低于荧光分光光度计的白色噪声水平。对于相同年龄的伊蚊或库蚊批次，记录到显著的荧光水平，但与其日历年龄无关。蚊子体内蝶啶的含量低，表明标准荧光分光光度法对此类研究不够灵敏。
Attempts were made to use pteridine fluorescence levels as a tool for aging individual Aedes polynesiensis Marks and Culex quinquefasciatus Say mosquitoes. Fluorescent pigments were extracted and quantified wit techniques and apparatus (i.e., a spectrofluorometer) that can be used in developing countries or in field laboratories, and that has already given consistent results in similar studies on other insect groups. However, for Ae. polynesiensis as well as Cx. quinquefasciatus, individual fluorescence measurements were mostly below the spectrofluoremter white noise level. With batches of Aedes or Culex of the same age, significant fluorescence levels were recorded but not in relation to their calendar ages. The low content of pteridines in mosquitoes suggests that standard spectrofluorometry is not sufficiently sensitive for such studies.