Quantitative measurement of oxygen metabolic rate in the rat brain using microPET imaging of briefly inhaled 15O-labelled oxygen gas.
Quantitative measurement of oxygen metabolic rate in the rat brain using microPET imaging of briefly inhaled 15O-labelled oxygen gas.
复制标题
使用短暂吸入 15O 标记氧气的 microPET 成像定量测量大鼠大脑中的氧代谢率。
DOI：
10.1097/01.mnm.0000220586.02591.fd
复制
发表时间：
2006
影响因子：
1.5
通讯作者：
Fox,PeterT
中科院分区：
文献类型：
--
作者：
Yee,Seong-Hwan;Lee,Kihak;Jerabek,PaulA;Fox,PeterT
目的用正电子发射断层扫描(PET)定量测量大鼠脑内氧代谢速率(CMRO2)一直是一个技术难题。方法在α-氯醛糖复合麻醉(30 mg·kg-1−1·h−-1，静脉滴注)条件下，用PET成像技术定量测定大鼠脑内CMRO2。在我们的实验中，15O标记的气体示踪剂(O15O)通过外科手术放置在气管内的插管向肺内注入。然后使用微型PET对示踪剂分布进行动态成像。与其他传统的PET方法不同，在这些方法中，需要提取一系列动脉血液样本来测量动脉输入功能，而不使用动脉血液样本。结果10只大鼠的CMRO2(μmol.10 0g−1·−1)为2 0 8±15(平均值±SD)。结论基于MicroPET的大鼠脑内CMRO2测量与动脉输入功能的非侵入性测量相结合具有很好的应用前景，特别是在许多涉及小动物的应用中，需要重复测量绝对CMRO2。
ObjectiveThe quantitative measurement of cerebral metabolic rate of oxygen (CMRO 2) for rats using positron emission tomography (PET) has been technically difficult. The present study was performed to provide a technique to measure CMRO 2 for rats using a dedicated animal PET technique.MethodsCMRO 2 in the rat brain was quantitatively measured under α-chloralose anaesthesia (30 mg· kg− 1· h− 1, intravenous infusion) using a PET imaging technique. In our experiment, the 15 O-labelled gas tracer (O 15 O) was administered by a bolus insufflation into the lung through a surgically placed cannula in the trachea. The tracer distribution was then dynamically imaged using the microPET. Unlike other conventional PET methods in which a series of arterial blood samples need to be withdrawn for the measurement of an arterial input function, no arterial blood sampling was employed. Instead, the heart was scanned in dynamic mode at the same time of imaging the brain, and the region of interest drawn over the heart was analysed to obtain an arterial input function.ResultsThe CMRO 2 value (μmol· 100 g− 1· min− 1) from 10 rats was 208±15 (mean±SD).ConclusionsOur results suggest that the microPET-based CMRO 2 measurement in the rat brain combined with a non-invasive measurement of arterial input function is promising, especially for many applications involving small animals in which repeated measurements of absolute CMRO 2 need to be performed.