Determination of the binding reaction between avidin and biotin by relaxation measurements of magnetic nanoparticles
Determination of the binding reaction between avidin and biotin by relaxation measurements of magnetic nanoparticles
复制标题
DOI：
10.1016/s0304-8853(98)00580-0
复制
发表时间：
1999-04-01
影响因子：
2.7
通讯作者：
Semmler, W
中科院分区：
文献类型：
--
作者：
Kötitz, R;Weitschies, W;Semmler, W
抗生物素蛋白和生物素化磁性氧化铁纳米颗粒之间的结合可以通过磁弛豫测量来监测。由颗粒聚集体引起的所得信号具有布朗分量和尼尔分量。布朗弛豫的时间常数和振幅由拟合算法确定，该算法也产生与样品的抗生物素蛋白含量成比例的Neel振幅。(C)1999 Elsevier Science B.V.保留所有权利。
The binding between avidin and biotinylated magnetic iron oxide nanoparticles can be monitored by means of magnetic relaxation measurements. The resultant signal, caused by particle aggregates, has Brownian and Neel components. The time constant and amplitude of Brownian relaxation were determined by a fit algorithm which also yielded Neel amplitudes proportional to the avidin content of the samples. (C) 1999 Elsevier Science B.V. All rights reserved.