Techniques for the collection of lymph from the liver, small intestine, or thoracic duct of the rat.
Techniques for the collection of lymph from the liver, small intestine, or thoracic duct of the rat.
复制标题
从大鼠肝脏、小肠或胸导管收集淋巴液的技术。
DOI：
10.5555/uri:pii:0022214348903369
复制
发表时间：
1948
期刊：
影响因子：
--
通讯作者：
J. H. Grindlay
中科院分区：
文献类型：
--
作者：
J. Bollman;J. Cain;J. H. Grindlay
从大鼠体内收集淋巴液的方法在从肝脏收集淋巴两到三天，从肠道或胸腔导管收集淋巴则长达十天，效果非常令人满意。这些方法在从血浆交换到淋巴的物质以及在各种食物的消化和吸收过程中从肠道产生淋巴的物质的角度研究淋巴是有用的。肝脏淋巴管的插管是在体重150到300克的大鼠身上完成的。大鼠用乙醚麻醉。通过正中切口，肝脏向上向右反射，胃和十二指肠向左反射，暴露出肝胃和肝十二指肠L韧带。伊文思蓝染料，0.1毫升的0.5%溶液，通过一根细小的皮下注射针注入肝脏。一分钟内，可在穿过肝胃韧带的淋巴管中看到蓝色淋巴。在Wurker获得了一些经验后，很容易识别出肝脏的清晰淋巴管，不再需要注射染料。使用解剖眼镜，钝性的D&I可以通过结扎小的
T HE following techniques for collection of lymph in the rat have been very satisfactory for periods of two or three days for lymph from the liver and for periods up to ten days for lymph from the intestine or from the thoracic duct. These methods have been useful in the study of lymph from the standpoint of the substances exchanged from the plasma to the lymph and of the materials contributed to the lymph from the intestine in the course of digestion and absorption of various foods. Cannulation of the lymphatics of the liver is accomplished in rats weighing 150 to 300 grams. The rats are anesthetized with ether. Through a midline incision the liver is reflected upward to the right and the stomach and duodenum are reflected to the left; this exposes the hepatogastric and hepatoduoden?l ligaments. Evans blue dye, 0.1 ml. of a 0.5 per cent solution, is injected through a fine hypodermic needle into the liver. Within a minute blue lymph may be seen in the lymphatics passing through the hepatogastric ligament. After the wurker has acquired a little experience the clear lymphatica of the liver are easily recognized and the injection of dye is no longer necessary. With the use of dissecting glasses, a blunt d&&i this can be prevented by ligation of the small