Evaluation of method for secondary DNA typing of Mycobacterium tuberculosis with pTBN12 in epidemiologic study of tuberculosis.
Evaluation of method for secondary DNA typing of Mycobacterium tuberculosis with pTBN12 in epidemiologic study of tuberculosis.
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pTBN12 结核分枝杆菌二次 DNA 分型方法在结核病流行病学研究中的评价。
DOI：
10.1128/jcm.34.12.3044-3048.1996
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发表时间：
1996
影响因子：
9.4
通讯作者：
Cave,MD
中科院分区：
文献类型：
--
作者：
Yang,Z;Chaves,F;Barnes,PF;Burman,WJ;Koehler,J;Eisenach,KD;Bates,JH;Cave,MD
研究发现，对于携带少量IS6110拷贝的菌株，使用包含pTBN12中存在的富含GC的多态重复序列的探针对结核分枝杆菌DNA进行二次指纹分析比使用插入序列IS6110进行指纹分析具有更强的区分力。为了验证pTBN12指纹在结核病分子流行病学中的应用，对来自美国和西班牙五个州的67名患者的结核分枝杆菌分离株进行了IS6110和pTBN12指纹分析。通过患者访谈和/或查阅病历来评估67名患者之间的流行病学联系。67个分离株有5个IS6110指纹图谱，其中2~5个拷贝的IS6110指纹图谱和18个pTBN12指纹图谱，其中10个由1个以上的菌株共有。在分离株具有相同pTBN12模式的患者中一致发现流行病学联系，而在分离株具有独特pTBN12模式的患者中没有发现联系。这表明，pTBN12指纹图谱是一种有用的工具，可以用来识别那些分离株具有相同的IS6110指纹的结核病患者，这些患者的IS6110指纹包含的片段少于6个。
Secondary fingerprinting of Mycobacterium tuberculosis DNA with a probe containing the polymorphic GC-rich repetitive sequence present in pTBN12 has been found to have greater discriminating power than does fingerprinting with the insertion sequence IS6110 for strains carrying few copies of IS6110. To validate the use of pTBN12 fingerprinting in the molecular epidemiology of tuberculosis, M. tuberculosis isolates from 67 patients in five states in the United States and in Spain were fingerprinted with both IS6110 and pTBN12. Epidemiologic links among the 67 patients were evaluated by patient interview and/or review of medical records. The 67 isolates had 5 IS6110 fingerprint patterns with two to five copies of IS6110 and 18 pTBN12 patterns, of which 10 were shared by more than 1 isolate. Epidemiologic links are consistently found among patients whose isolates had identical pTBN12 patterns, whereas no links were found among patients whose isolates had unique pTBN12 patterns. This suggests that pTBN12 fingerprinting is a useful tool to identify epidemiologically linked tuberculosis patients whose isolates have identical IS6110 fingerprints containing fewer than six fragments.