Atypical adenomatous hyperplasia and bronchoalveolar lung carcinoma - Analysis by morphometry and the expressions of p53 and carcinoembryonic antigen
Atypical adenomatous hyperplasia and bronchoalveolar lung carcinoma - Analysis by morphometry and the expressions of p53 and carcinoembryonic antigen
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DOI：
10.1097/00000478-199605000-00002
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发表时间：
1996-05-01
影响因子：
5.6
通讯作者：
Kanisawa, M
中科院分区：
文献类型：
--
作者：
Kitamura, H;Kameda, Y;Kanisawa, M
肺的非典型腺瘤样增生（AAH）是支气管肺泡癌（BAC）的一个假定的前兆。为了确定其发展的步骤，并澄清在哪个阶段发生的关键细胞事件，我们研究了65个病变的AAH，早期BAG，和明显的BAC的形态分析和免疫组化评价的p53蛋白和癌胚抗原（CEA）的表达。从AAH到早期BAC和明显BAG，核面积和病变大小均增加，核面积的标准化变异在明显BAG中最小。判别分析，使用这些形态参数显示高准确率为各自的类别。根据核面积和病变大小分析肺部病变的分布，获得了区分AAH和早期BAG的有效、潜在的诊断临界值。p53和CEA的表达均随乳腺癌分级的增高而增高。特别是，高水平的p53表达与明显的BAG密切相关。这些发现表明，我们的肺部病变的分类是可重复的，因此有助于分析BAG的发展。此外，与高水平p53表达相关的某些类型的p53基因异常可能在早期至显性BAC的进展中起重要作用。
Atypical adenomatous hyperplasia (AAH) of the lung is a putative precursor of bronchoalveolar carcinoma (BAC). To define the steps in its development and to clarify at which stage critical cellular events occur, we studied 65 lesions of AAH, early BAG, and overt BAC by morphometric analysis and immunohistochemical evaluation of expression of p53 protein and carcinoembryonic antigen (CEA). Both the nuclear area and lesion size increased from AAH to early BAC and to overt BAG; the standardized variation of nuclear area was smallest in overt BAG. Discriminant analysis using these morphometric parameters revealed high accuracy rates for the respective categories. Analysis of distribution of lung lesions in terms of nuclear area and lesion size yielded effective, potentially diagnostic cutoff values for distinction between AAH and early BAG. Both p53 and CEA expression tended to increase with the advance of atypia grade. In particular, high-level p53 expression was strongly correlated with overt BAG. These findings indicate that our classification of lung lesions is reproducible and thus useful for analyzing the development of BAG. Furthermore, some kinds of p53 gene abnormalities that are correlated with high-level p53 expression likely play an important role in the progression of early to overt BAC.