Spectrometer-free vibrational imaging by retrieving stimulated Raman signal from highly scattered photons.
Spectrometer-free vibrational imaging by retrieving stimulated Raman signal from highly scattered photons.
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DOI：
10.1126/sciadv.1500738
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发表时间：
2015-10
期刊：
影响因子：
13.6
通讯作者：
Cheng JX
中科院分区：
文献类型：
--
作者：
Liao CS;Wang P;Wang P;Li J;Lee HJ;Eakins G;Cheng JX
振动成像揭示了维生素E在小鼠体内皮肤上的分布，并原位捕获了人类乳腺癌组织。在体内振动光谱成像被抑制的第二规模上的相对缓慢的光谱采集和低的光子收集效率的高度散射系统。近年来发展起来的多路相干反斯托克斯拉曼散射和受激拉曼散射技术将光谱采集时间提高到微秒级。使用分光计设置的这些方法不适合于几乎所有光子都散射的混浊系统。我们演示了通过入射光子的空间频率复用和单个光电二极管检测60 μs内的受激拉曼光谱的振动成像。与光谱仪的设置相比，我们的方法提高了两个数量级的光子收集效率的高散射标本。我们展示了小鼠皮肤上维生素E分布的体内成像和人类乳腺癌组织的原位成像。报道的工作开辟了新的机会，光谱成像在手术室和发展的深层组织拉曼光谱向分子水平的诊断。
Vibrational imaging reveals vitamin E distribution on mouse skin in vivo and captures human breast cancer tissues in situ. In vivo vibrational spectroscopic imaging is inhibited by relatively slow spectral acquisition on the second scale and low photon collection efficiency for a highly scattering system. Recently developed multiplex coherent anti-Stokes Raman scattering and stimulated Raman scattering techniques have improved the spectral acquisition time down to microsecond scale. These methods using a spectrometer setting are not suitable for turbid systems in which nearly all photons are scattered. We demonstrate vibrational imaging by spatial frequency multiplexing of incident photons and single photodiode detection of a stimulated Raman spectrum within 60 μs. Compared to the spectrometer setting, our method improved the photon collection efficiency by two orders of magnitude for highly scattering specimens. We demonstrated in vivo imaging of vitamin E distribution on mouse skin and in situ imaging of human breast cancerous tissues. The reported work opens new opportunities for spectroscopic imaging in a surgical room and for development of deep-tissue Raman spectroscopy toward molecular level diagnosis.